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Western Blot (WB)
(Western Blot: Sample: Mouse Cardiac Muscle lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse UCP2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Uncoupling Protein 2, Mitochondrial (UCP2) Polyclonal Antibody | anti-UCP2 antibody
Polyclonal Antibody to Uncoupling Protein 2, Mitochondrial (UCP2)
Gene Names
Ucp2; UCPH; UCP 2; Slc25a8
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Uncoupling Protein 2; Mitochondrial (UCP2); Polyclonal Antibody; Polyclonal Antibody to Uncoupling Protein 2; anti-UCP2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against UCP2. It has been selected for its ability to recognize UCP2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-A GGGRRYQSTV EAYKTIAREE GIRGLWKGTS PNVARNAIVN CAELVTYDLI KDTLLKANLM TDDLPCHFTS AFGAGFCTTV IASPVDVVKT RYMNSALGQY HSAGHCALTM LRKEGPRAFY KGFMPSFLRL GSWNVVMFVT YEQLKRALMA AYQS
MGHHHHHHSGSEF-A GGGRRYQSTV EAYKTIAREE GIRGLWKGTS PNVARNAIVN CAELVTYDLI KDTLLKANLM TDDLPCHFTS AFGAGFCTTV IASPVDVVKT RYMNSALGQY HSAGHCALTM LRKEGPRAFY KGFMPSFLRL GSWNVVMFVT YEQLKRALMA AYQS
Sequence Length
309
Applicable Applications for anti-UCP2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant UCP2 (Ala150~Ser304) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2066889
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Cardiac Muscle lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse UCP2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Cardiac Muscle lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse UCP2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,374 Da
NCBI Official Full Name
mitochondrial uncoupling protein 2
NCBI Official Synonym Full Names
uncoupling protein 2 (mitochondrial, proton carrier)
NCBI Official Symbol
Ucp2
NCBI Official Synonym Symbols
UCPH; UCP 2; Slc25a8
NCBI Protein Information
mitochondrial uncoupling protein 2
UniProt Protein Name
Mitochondrial uncoupling protein 2
UniProt Gene Name
Ucp2
UniProt Synonym Gene Names
Slc25a8; UCP 2
Uniprot Description
UCP2: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Mitochondrial; Transporter
Chromosomal Location of Human Ortholog: 7 E2|7 54.36 cM
Cellular Component: cytoplasm; integral component of membrane; membrane; mitochondrial inner membrane; mitochondrial membrane; mitochondrion
Molecular Function: oxidative phosphorylation uncoupler activity
Biological Process: mitochondrial transport; negative regulation of apoptosis; regulation of mitochondrial membrane potential; response to cold; response to hypoxia; response to superoxide; transport
Research Articles on UCP2
Similar Products
Product Notes
The UCP2 ucp2 (Catalog #AAA2002389) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Uncoupling Protein 2, Mitochondrial (UCP2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Uncoupling Protein 2, Mitochondrial (UCP2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the UCP2 ucp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-A GGGRRYQSTV EAYKTIAREE GIRGLWKGTS PNVARNAIVN CAELVTYDLI KDTLLKANLM TDDLPCHFTS AFGAGFCTTV IASPVDVVKT RYMNSALGQY HSAGHCALTM LRKEGPRAFY KGFMPSFLRL GSWNVVMFVT YEQLKRALMA AYQS. It is sometimes possible for the material contained within the vial of "Uncoupling Protein 2, Mitochondrial (UCP2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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