Rabbit anti-Human UNC5C Polyclonal Antibody | anti-UNC5C antibody

UNC5C (unc-5 homolog C, Netrin receptor UNC5C precursor, UNC5H3, Unc-5 homolog 3, Unc-5 homolog C) (Biotin)

Cat. Num. AAA6504454

• Gene Names: UNC5C; UNC5H3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: UNC5C; Polyclonal Antibody; UNC5C (unc-5 homolog C; Netrin receptor UNC5C precursor; UNC5H3; Unc-5 homolog 3; Unc-5 homolog C) (Biotin); anti-UNC5C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UNC5C.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-UNC5C antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human UNC5C (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of UNC5C Antibody (Center) in K562 cell line lysates (35ug/lane). UNC5C (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue with UNC5C Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of K562 cells using UNC5C Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-UNC5C antibody

Antibodies; Netrin

References

1. Hibi, K., et.al., World J Surg 33(5), 1053-1057 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 16933525
• NCBI GeneID: 8633
• NCBI Accession #: NP_003719.2
• NCBI GenBank Nucleotide #: NM_003728.2
• UniProt Accession #: O95185; Q8IUT0
• Molecular Weight: 66,079 Da
• NCBI Official Full Name: netrin receptor UNC5C
• NCBI Official Synonym Full Names: unc-5 homolog C (C. elegans)
• NCBI Official Symbol: UNC5C
• NCBI Official Synonym Symbols: UNC5H3
• NCBI Protein Information: netrin receptor UNC5C; unc-5 homolog 3; protein unc-5 homolog 3; protein unc-5 homolog C; unc5 (C.elegans homolog) c
• UniProt Protein Name: Netrin receptor UNC5C
• Protein Family: UNC5C-like protein
• UniProt Gene Name: UNC5C
• UniProt Synonym Gene Names: UNC5H3
• UniProt Entry Name: UNC5C_HUMAN

NCBI Description

This gene product belongs to the UNC-5 family of netrin receptors. Netrins are secreted proteins that direct axon extension and cell migration during neural development. They are bifunctional proteins that act as attractants for some cell types and as repellents for others, and these opposite actions are thought to be mediated by two classes of receptors. The UNC-5 family of receptors mediate the repellent response to netrin; they are transmembrane proteins containing 2 immunoglobulin (Ig)-like domains and 2 type I thrombospondin motifs in the extracellular region. [provided by RefSeq, Jul 2008]

Uniprot Description

UNC5C: Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. Also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Belongs to the unc-5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 4q21-q23

Cellular Component: integral to membrane; plasma membrane

Molecular Function: netrin receptor activity

Biological Process: axon guidance; apoptosis; positive regulation of apoptosis; brain development; regulation of cell migration; anterior/posterior axon guidance