Rabbit anti-Human ULK1 Polyclonal Antibody | anti-ULK1 antibody

ULK1 polyclonal antibody

Cat. Num. AAA3015530

• Gene Names: ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Synonyms: ULK1; Polyclonal Antibody; ULK1 polyclonal antibody; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; ATG1; hATG1; Unc-51-like kinase 1; KIAA0722; anti-ULK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: ULK1 polyclonal antibody detects endogenous levels of ULK1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Applicable Applications for anti-ULK1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000
• Immunogen: Synthetic peptide, corresponding to Human ULK1.
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of ULK1 polyclonal antibody at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:H1792 whole cell lysate(40ug))

Related Product Information for anti-ULK1 antibody

Two related serine/threonine kinases, UNC-51-like kinase 1 and 2 (ULK1, ULK2), were discovered as mammalian homologs of the C. elegans gene UNC-51 in which mutants exhibited abnormal axonal extension and growth. Both proteins are widely expressed and contain an amino-terminal kinase domain followed by a central proline/serine rich domain and a highly conserved carboxy-terminal domain. The roles of ULK1 and ULK2 in axon growth have been linked to studies showing that the kinases are localized to neuronal growth cones and are involved in endocytosis of critical growth factors, such as NGF. Yeast two-hybrid studies found ULK1/2 associated with modulators of the endocytic pathway, SynGAP and syntenin. Structural similarity of ULK1/2 has also been recognized with the yeast autophagy protein Atg1/Apg1. Knockdown experiments using siRNA demonstrated that ULK1 is essential for autophagy, a catabolic process for the degradation of bulk cytoplasmic contents. It appears that Atg1/ULK1 can act as a convergence point for multiple signals that control autophagy, and can bind to several autophagy-related (Atg) proteins, regulating phosphorylation states and protein trafficking. AMPK, activated during low nutrient conditions, directly phosphorylates ULK1 at multiple sites including Ser317, Ser555, and Ser777. Conversely, mTOR, which is a regulator of cell growth and an inhibitor of autophagy, phosphorylates ULK1 at Ser757 and disrupts the interaction between ULK1 and AMPK.

NCBI and Uniprot Product Information

• NCBI GI #: 4507831
• NCBI GeneID: 8408
• NCBI Accession #: NP_003556
• NCBI GenBank Nucleotide #: NM_003565.2
• UniProt Accession #: O75385; Q9UQ28
• Molecular Weight: Predicted: 116 kDa; ; Observed: 105 kDa
• NCBI Official Full Name: serine/threonine-protein kinase ULK1
• NCBI Official Synonym Full Names: unc-51 like autophagy activating kinase 1
• NCBI Official Symbol: ULK1
• NCBI Official Synonym Symbols: ATG1; ATG1A; UNC51; hATG1; Unc51.1
• NCBI Protein Information: serine/threonine-protein kinase ULK1; ATG1 autophagy related 1 homolog; autophagy-related protein 1 homolog
• UniProt Protein Name: Serine/threonine-protein kinase ULK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: ULK1
• UniProt Synonym Gene Names: KIAA0722; ATG1; hATG1
• UniProt Entry Name: ULK1_HUMAN

Uniprot Description

ULK1: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. Interacts with GABARAP and GABARAPL2. Interacts (via C- terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation. Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

Protein type: Protein kinase, Other; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Autophagy; Other group; ULK family

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: neuron projection; cytoplasmic vesicle membrane; cell soma; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein complex binding; protein kinase binding; ATP binding; Rab GTPase binding

Biological Process: axon extension; protein amino acid autophosphorylation; neurite regeneration; negative regulation of collateral sprouting; cerebellar granule cell differentiation; protein amino acid phosphorylation; radial glia guided migration of granule cell; response to starvation; cellular response to nutrient levels; protein localization; receptor internalization; Ras protein signal transduction; regulation of autophagy; regulation of nerve growth factor receptor signaling pathway; positive regulation of macroautophagy; positive regulation of autophagy; neurite development; autophagic vacuole formation