Rabbit anti-Human ULBP1 Polyclonal Antibody | anti-ULBP1 antibody

Anti-ULBP1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108417

• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: ULBP1; Polyclonal Antibody; Anti-ULBP1 Antibody; Rabbit Polyclonal; ULBP1 Antibody; Rabbit PAb; Antigen Affinity Purified; UL16 binding protein 1; Anti-RAET1I Antibody; anti-ULBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ULBP1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-ULBP1 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:50-1:200; ICC/IF: 1:100-1:500
• Immunogen: E Coli-derived Human ULBP1 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human ULBP1 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human ULBP1 in human testis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of ULBP1 in Hek293 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human ULBP1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Related Product Information for anti-ULBP1 antibody

UL16-binding proteins (ULBP) or retinoic acid early transcripts-1 (RAET1) are ligands to the activating receptor, NKG2D. Ten members of the human ULBP/RAET1 gene family have been identified to encode for potentially functional proteins, and have tissue-specific expressions. ULBP1, also known as RAET1I and NKG2DL1, together with at least ULBP 2 and 3, are well-known ligands for NKG2D, and activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. ULBP1 is expressed in T-cells, B-cells, erythroleukemia cell lines and in a wide range of tissues including heart, brain, lung, liver and bone marrow, as well as some tumor cells. As an unconventional member of the MHC class I family, ULBP1 function in immune responses, especially in cancer and infectious diseases. Unlike other ULBP members, ULBP1 is able to interact with soluble CMV glycoprotein UL16 in CMV infected cells. The interaction with UL16 blocked the interaction with the NKG2D receptor, and thus might escape the immune surveillance. Furthermore, UL16 also causes ULBP1 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. The ULBP1 regulation may have implications for development of new therapeutic strategies against cancer cells.

References

1. Rolle, A. et al., 2003, J Immunol. 171(2): 902-908. 2. Lopez-Soto, A. et al., 2006, J Biol Chem. 281(41): 30419-30430. 3. Song, H. et al., 2006, Cell Immunol. 239(1): 22-30. 4. Eisele, G. et al., 2006, Brain. 129 (9): 2416-2425. 5. Romphruk, AV. et al., 2009, Immunogenetics. 61(9): 611-617. 6. Sutherland, C.L. et. al., 2002, J. Immunol. 168: 671-679.