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Western Blot (WB) (All lanes : Anti-UGT1A9 Antibody (N-Term) at 1:2000 dilutionLane 1: human liver lysateLane 2: mouse liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human, Mouse UGT1A9 Polyclonal Antibody | anti-UGT1A9 antibody

UGT1A9 Antibody (N-Term)

Gene Names
UGT1A9; LUGP4; UDPGT; UGT1I; HLUGP4; UGT-1I; UGT1-9; UGT1.9; UGT1AI; UGT1-09; UGT1A9S; UDPGT 1-9
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Synonyms
UGT1A9; Polyclonal Antibody; UGT1A9 Antibody (N-Term); UDP-glucuronosyltransferase 1-9; UDPGT 1-9; UGT1*9; UGT1-09; UGT1.9; 2.4.1.17; UDP-glucuronosyltransferase 1-I; UGT-1I; UGT1I; UDP-glucuronosyltransferase 1A9; lugP4; GNT1; UGT1; anti-UGT1A9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This UGT1A9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 62-92 amino acids from human UGT1A9.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
530
Applicable Applications for anti-UGT1A9 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:2000
Antigen Type
Synthesized Peptide
Antigen Source
Human
Cellular Location
Microsome. Endoplasmic reticulum membrane; Single-pass membrane protein
Tissue Location
Liver. Isoform 1 and isoform 2 are expressed in liver, kidney, colon, esophagus and small intestine
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-UGT1A9 Antibody (N-Term) at 1:2000 dilutionLane 1: human liver lysateLane 2: mouse liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-UGT1A9 Antibody (N-Term) at 1:2000 dilutionLane 1: human liver lysateLane 2: mouse liver lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-UGT1A9 antibody
UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform has specificity for phenols. Isoform 2 lacks transferase activity but acts as a negative regulator of isoform 1.
Product Categories/Family for anti-UGT1A9 antibody
References
Wooster R.,et al.Biochem. J. 278:465-469 (1991). Ciotti M.,et al.Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases. Gong Q.H.,et al. Pharmacogenetics 11:357-368 (2001). Hillier L.W.,et al.Nature 434:724-731 (2005). Owens I.S.,et al.Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59941 Da
NCBI Official Full Name
UDP-glucuronosyltransferase 1-9
NCBI Official Synonym Full Names
UDP glucuronosyltransferase family 1 member A9
NCBI Official Symbol
UGT1A9
NCBI Official Synonym Symbols
LUGP4; UDPGT; UGT1I; HLUGP4; UGT-1I; UGT1-9; UGT1.9; UGT1AI; UGT1-09; UGT1A9S; UDPGT 1-9
NCBI Protein Information
UDP-glucuronosyltransferase 1-9
UniProt Protein Name
UDP-glucuronosyltransferase 1-9
UniProt Gene Name
UGT1A9
UniProt Synonym Gene Names
GNT1; UGT1; UDPGT 1-9; UGT1*9; UGT1-09; UGT1.9; UGT-1I; UGT1I

NCBI Description

This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The enzyme encoded by this gene is active on phenols. [provided by RefSeq, Jul 2008]

Uniprot Description

UGT1A9: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform has specificity for phenols. Belongs to the UDP-glycosyltransferase family. 1 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - starch and sucrose; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Cofactor and Vitamin Metabolism - retinol; EC 2.4.1.17; Endoplasmic reticulum; Lipid Metabolism - androgen and estrogen; Membrane protein, integral; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - drug metabolism - other enzymes; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane

Molecular Function: glucuronosyltransferase activity; protein heterodimerization activity; protein homodimerization activity; retinoic acid binding

Biological Process: flavone metabolic process; metabolic process; retinoic acid metabolic process; xenobiotic metabolic process

Research Articles on UGT1A9

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Product Notes

The UGT1A9 ugt1a9 (Catalog #AAA9231008) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UGT1A9 Antibody (N-Term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UGT1A9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:2000. Researchers should empirically determine the suitability of the UGT1A9 ugt1a9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UGT1A9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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