Western Blot (WB)
(WB Suggested Anti-UGT1A4 Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysate)
Rabbit anti-Human UGT1A4 Polyclonal Antibody | anti-UGT1A4 antibody
UGT1A4 antibody - N-terminal region
Western Blot (WB)
(WB Suggested Anti-UGT1A4 Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysate)
Western Blot (WB)
(WB Suggested Anti-UGT1A4 Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysate)
Target Description: UGT1A4 is an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, hormones, and drugs, into water-soluble, excretable metabolites. This enzyme has some glucuronidase activity towards amines, steroids, and sapogenins. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. This enzyme has some glucuronidase activity towards bilirubin, although is is more active on amines, steroids, and sapogenins. [provided by RefSeq, Jul 2008]
Uniprot Description
UGT1A4: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX- alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Defects in UGT1A4 are the cause of Gilbert syndrome (GILBS). Gilbert syndrome occurs as a consequence of reduced bilirubin transferase activity and is often detected in young adults with vague nonspecific complaints. Defects in UGT1A4 are the cause of Crigler-Najjar syndrome type 1 (CN1). CN1 patients have severe hyperbilirubinemia and usually die of kernicterus (bilirubin accumulation in the basal ganglia and brainstem nuclei) within the first year of life. CN1 inheritance is autosomal recessive. Defects in UGT1A4 are the cause of Crigler-Najjar syndrome type 2 (CN2). CN2 patients have less severe hyperbilirubinemia and usually survive into adulthood without neurologic damage. Phenobarbital, which induces the partially deficient glucuronyl transferase, can diminish the jaundice. CN2 inheritance is autosomal dominant. Belongs to the UDP-glycosyltransferase family. 1 isoforms of the human protein are produced by alternative splicing.
Protein type: Carbohydrate Metabolism - pentose and glucuronate interconversions; Cofactor and Vitamin Metabolism - retinol; EC 2.4.1.17; Lipid Metabolism - androgen and estrogen; Xenobiotic Metabolism - drug metabolism - other enzymes; Membrane protein, integral; Transferase; Carbohydrate Metabolism - starch and sucrose; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Carbohydrate Metabolism - ascorbate and aldarate; Endoplasmic reticulum; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450
Chromosomal Location of Human Ortholog: 2q37
Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane
Molecular Function: enzyme binding; protein homodimerization activity; retinoic acid binding; protein heterodimerization activity; glucuronosyltransferase activity
Biological Process: negative regulation of transferase activity; flavonoid biosynthetic process; heme catabolic process; xenobiotic metabolic process; porphyrin metabolic process; negative regulation of fatty acid metabolic process; drug metabolic process; cellular response to hormone stimulus
Disease: Gilbert Syndrome; Crigler-najjar Syndrome, Type I
Research Articles on UGT1A4
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Product Notes
The UGT1A4 ugt1a4 (Catalog #AAA3208348) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UGT1A4 antibody - N-terminal region reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UGT1A4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the UGT1A4 ugt1a4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: VVLTPEVNMH IKEEKFFTLT AYAVPWTQKE FDRVTLGYTQ GFFETEHLLK. It is sometimes possible for the material contained within the vial of "UGT1A4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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