Western Blot (WB)
(UGT1A1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane). This demonstrates the UGT1A1 antibody detected the UGT1A1 protein (arrow).)
Rabbit anti-Human UGT1A1 Polyclonal Antibody | anti-UGT1A1 antibody
UGT1A1 Antibody (N-term)
IF~~1:10~50
IHC-P~~1:10~50
Western Blot (WB)
(UGT1A1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane). This demonstrates the UGT1A1 antibody detected the UGT1A1 protein (arrow).)
Western Blot (WB)
(UGT1A1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane). This demonstrates the UGT1A1 antibody detected the UGT1A1 protein (arrow).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of UGT1A1 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of UGT1A1 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunohistochemistry (IHC)
(UGT1A1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of UGT1A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(UGT1A1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of UGT1A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Justenhoven, C., et al. Breast Cancer Res. Treat. 124(1):289-292(2010)
Hu, M., et al. Pharmacogenet. Genomics 20(10):634-637(2010)
Sai, K., et al. Br J Clin Pharmacol 70(2):222-233(2010)
Kilic, I., et al. Int J Clin Pharmacol Ther 48(8):504-508(2010)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome. [provided by RefSeq, Jul 2008]
Uniprot Description
UGT1A1: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX- alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Is also able to catalyze the glucuronidation of 17beta-estradiol, 17alpha-ethinylestradiol, 1-hydroxypyrene, 4- methylumbelliferone, 1-naphthol, paranitrophenol, scopoletin, and umbelliferone. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Expressed in liver. Not expressed in skin or kidney. Belongs to the UDP-glycosyltransferase family. 1 isoforms of the human protein are produced by alternative splicing.
Protein type: Cofactor and Vitamin Metabolism - retinol; Carbohydrate Metabolism - starch and sucrose; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Lipid Metabolism - androgen and estrogen; Carbohydrate Metabolism - ascorbate and aldarate; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Transferase; Membrane protein, integral; Xenobiotic Metabolism - metabolism by cytochrome P450; Carbohydrate Metabolism - pentose and glucuronate interconversions; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 2.4.1.17
Chromosomal Location of Human Ortholog: 2q37
Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to plasma membrane
Molecular Function: enzyme inhibitor activity; retinoic acid binding; enzyme binding; protein homodimerization activity; glucuronosyltransferase activity; protein heterodimerization activity; steroid binding
Biological Process: steroid metabolic process; response to drug; estrogen metabolic process; organ regeneration; negative regulation of steroid metabolic process; response to lipopolysaccharide; negative regulation of fatty acid metabolic process; liver development; cellular response to hormone stimulus; response to starvation; negative regulation of transferase activity; flavonoid biosynthetic process; bilirubin conjugation; heme catabolic process; xenobiotic metabolic process; digestion; porphyrin metabolic process; negative regulation of catalytic activity; flavone metabolic process; acute-phase response; retinoic acid metabolic process; heterocycle metabolic process; drug metabolic process; response to nutrient
Disease: Gilbert Syndrome; Crigler-najjar Syndrome, Type I; Bilirubin, Serum Level Of, Quantitative Trait Locus 1; Crigler-najjar Syndrome, Type Ii; Hyperbilirubinemia, Transient Familial Neonatal
Research Articles on UGT1A1
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Product Notes
The UGT1A1 ugt1a1 (Catalog #AAA9203265) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 65-90. The UGT1A1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UGT1A1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB). WB~~1:1000 IF~~1:10~50 IHC-P~~1:10~50. Researchers should empirically determine the suitability of the UGT1A1 ugt1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UGT1A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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