Rabbit anti-Human UGT1A1 Polyclonal Antibody | anti-UGT1A1 antibody

UGT1A1, NT (UGT1A1, GNT1, UGT1, UDP-glucuronosyltransferase 1-1, Bilirubin-specific UDPGT isozyme 1, UDP-glucuronosyltransferase 1-A, UDP-glucuronosyltransferase 1A1)

Cat. Num. AAA6001505

• Gene Names: UGT1A1; GNT1; UGT1; UDPGT; UGT1A; HUG-BR1; BILIQTL1; UDPGT 1-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: UGT1A1; Polyclonal Antibody; NT (UGT1A1; GNT1; UGT1; UDP-glucuronosyltransferase 1-1; Bilirubin-specific UDPGT isozyme 1; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A1); Anti -UGT1A1; anti-UGT1A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-UGT1A1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunofluorescence: 1:10-50; Immunohistochemistry: 1:10-50
• Immunogen: UGT1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-91 amino acids from the N-terminal region of human UGT1A1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) using MBS6001505. This demonstrates that MBS6001505 detected the UGT1A1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS6001505 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6001505 for immunohistochemistry.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis with HepG2 cell using MBS6001505 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-UGT1A1 antibody

This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome.

Product Categories/Family for anti-UGT1A1 antibody

Antibodies; Abs to Enzymes; Glucuronosyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 8850236
• NCBI GeneID: 54658
• NCBI Accession #: NP_000454.1
• NCBI GenBank Nucleotide #: NM_000463.2
• UniProt Accession #: P22309
• Molecular Weight: 59,591 Da
• NCBI Official Full Name: UDP-glucuronosyltransferase 1-1
• NCBI Official Synonym Full Names: UDP glucuronosyltransferase 1 family, polypeptide A1
• NCBI Official Symbol: UGT1A1
• NCBI Official Synonym Symbols: GNT1; UGT1; UDPGT; UGT1A; HUG-BR1; BILIQTL1; UDPGT 1-1
• NCBI Protein Information: UDP-glucuronosyltransferase 1-1; UGT-1A; UGT1*1; UGT1-01; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A1; bilirubin-specific UDPGT isozyme 1; bilirubin UDP-glucuronosyltransferase 1-1; bilirubin UDP-glucuronosyltransferase isozyme 1; UDP glycosyltransferase 1 family, polypeptide A1
• UniProt Protein Name: UDP-glucuronosyltransferase 1-1
• Protein Family: UDP-glucuronosyltransferase
• UniProt Gene Name: UGT1A1
• UniProt Synonym Gene Names: GNT1; UGT1; UDPGT 1-1; UGT1*1; UGT1-01; UGT1.1; hUG-BR1; UGT-1A; UGT1A
• UniProt Entry Name: UD11_HUMAN

NCBI Description

This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

UGT1A1: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX- alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Is also able to catalyze the glucuronidation of 17beta-estradiol, 17alpha-ethinylestradiol, 1-hydroxypyrene, 4- methylumbelliferone, 1-naphthol, paranitrophenol, scopoletin, and umbelliferone. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Expressed in liver. Not expressed in skin or kidney. Belongs to the UDP-glycosyltransferase family. 1 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - ascorbate and aldarate; Lipid Metabolism - androgen and estrogen; Carbohydrate Metabolism - starch and sucrose; Membrane protein, integral; EC 2.4.1.17; Carbohydrate Metabolism - pentose and glucuronate interconversions; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - other enzymes; Cofactor and Vitamin Metabolism - retinol; Transferase

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; endoplasmic reticulum

Molecular Function: enzyme inhibitor activity; protein homodimerization activity; enzyme binding; retinoic acid binding; protein heterodimerization activity; glucuronosyltransferase activity; steroid binding

Biological Process: response to drug; steroid metabolic process; estrogen metabolic process; organ regeneration; negative regulation of steroid metabolic process; response to lipopolysaccharide; negative regulation of fatty acid metabolic process; liver development; cellular response to hormone stimulus; response to starvation; negative regulation of transferase activity; flavonoid biosynthetic process; bilirubin conjugation; heme catabolic process; xenobiotic metabolic process; negative regulation of catalytic activity; digestion; porphyrin metabolic process; acute-phase response; flavone metabolic process; retinoic acid metabolic process; heterocycle metabolic process; drug metabolic process; response to nutrient

Disease: Gilbert Syndrome; Crigler-najjar Syndrome, Type I; Bilirubin, Serum Level Of, Quantitative Trait Locus 1; Hyperbilirubinemia, Transient Familial Neonatal; Crigler-najjar Syndrome, Type Ii