Mouse anti-Human FAN1 Polyclonal Antibody | anti-FAN1 antibody

FAN1 (MTMR15, Fanconi-associated Nuclease 1, FANCD2/FANCI-associated Nuclease 1, Myotubularin-related Protein 15, KIAA1018, MTMR15)

Cat. Num. AAA6011372

• Gene Names: FAN1; KMIN; MTMR15; KIAA1018
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: FAN1; Polyclonal Antibody; FAN1 (MTMR15; Fanconi-associated Nuclease 1; FANCD2/FANCI-associated Nuclease 1; Myotubularin-related Protein 15; KIAA1018; MTMR15); Anti -FAN1 (MTMR15; anti-FAN1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MTMR15.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MSEGKPPDKKKRPRRSLSISKNKKKASNSIISCFNNAPPAKLACPVCSKMVPRYDLNRHLDEMCANNDFVQVDPGQVGLINSNVSMVDLTSVTLEDVTPKKSPPPKTNLTPGQSDSAKREVKQKISPYFKSNDVVCKNQDELRNRSVKVICLGSLASKLSRKYVKAKKSIDKDEEFAGSSPQSSKSTVVKSLIDNSSEIEDEDQILENSSQKENVFKCDSLKEECIPEHMVRESKIMEAESQKATRECEKSALTPGFSDNAIMLFSPDFTLRNTLKSTSEDSLVKQECIKEVVEKREACHCEEVKMTVASEAKIQLSDSEAKSHSSADDASAWSNIQEAPLQDDSCLNNDIPHSIPLEQGSSCNGPGQTTGHPYYLRSFLVVLKTVLENEDDMLLFDEQEKGIVTKFYQLSATGQKLYVRLFQRKLSWIKMTKLEYEEIALDLTPVIEELTNAGFLQTESELQELSEVLELLSAPELKSLAKTFHLVNPNGQKQQLVDAFLKLAKQRSVCTWGKNKPGIGAVILKRFCWLLLQ

• Applicable Applications for anti-FAN1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human MTMR15, aa1-533 (AAH47882.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KIAA1018 polyclonal antibody. Western Blot analysis of KIAA1018 expression in human spleen)

Western Blot (WB)

(Western Blot analysis of KIAA1018 expression in transfected 293T cell line by KIAA1018 polyclonal antibody. Lane 1: KIAA1018 transfected lysate (58.63kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to MTMR15 on HeLa cell. [antibody concentration 10ug/ml])

Related Product Information for anti-FAN1 antibody

Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap-specific enzymes to unhook the ICL by cleaving the lagging-strand template.

Product Categories/Family for anti-FAN1 antibody

Antibodies; Abs to Protein Phosphatases

NCBI and Uniprot Product Information

• NCBI GI #: 226246529
• NCBI GeneID: 22909
• NCBI Accession #: NP_001139568.1
• NCBI GenBank Nucleotide #: NM_001146096.1
• UniProt Accession #: Q9Y2M0; Q86WU8; A8K4M2
• Molecular Weight: 114,225 Da
• NCBI Official Full Name: fanconi-associated nuclease 1 isoform b
• NCBI Official Synonym Full Names: FANCD2/FANCI-associated nuclease 1
• NCBI Official Symbol: FAN1
• NCBI Official Synonym Symbols: KMIN; MTMR15; KIAA1018
• NCBI Protein Information: fanconi-associated nuclease 1; myotubularin-related protein 15; fanconi anemia associated nuclease 1; coiled-coil domain-containing protein MTMR15
• UniProt Protein Name: Fanconi-associated nuclease 1
• Protein Family: Fanconi-associated nuclease
• UniProt Gene Name: FAN1
• UniProt Synonym Gene Names: KIAA1018; MTMR15
• UniProt Entry Name: FAN1_HUMAN

NCBI Description

This gene encodes a member of the myotubularin-related class 1 cysteine-based protein tyrosine phosphatases. The encoded protein may be catalytically inactive. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2009]

Uniprot Description

FAN1: Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL- induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap- specific enzymes to unhook the ICL by cleaving the lagging-strand template. Belongs to the FAN1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Deoxyribonuclease; EC 3.1.4.1; Ubiquitin conjugating system; DNA repair, damage

Chromosomal Location of Human Ortholog: 15q13.2-q13.3

Cellular Component: nucleoplasm; intercellular bridge; cytoplasm; nucleus

Molecular Function: 5'-3' exonuclease activity; protein binding; phosphodiesterase I activity; ubiquitin binding; 5'-flap endonuclease activity; magnesium ion binding

Biological Process: nucleotide-excision repair; nucleotide-excision repair, DNA incision; DNA repair; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination

Disease: Interstitial Nephritis, Karyomegalic