Western Blot (WB)
(All lanes : Anti-MYL1 Antibody (N-Term) at 1:2000 dilutionLane 1: Human skeletal muslce lysateLane 2: Rat skeletal muscle lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, Rat MYL1 Polyclonal Antibody | anti-MYL1 antibody
MYL1 Antibody (N-Term)
Gene Names
MYL1; MLC1F; MLC3F
Reactivity
Human, Rat
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Synonyms
MYL1; Polyclonal Antibody; MYL1 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Myosin light chain 1/3; skeletal muscle isoform; MLC1/MLC3; MLC1F/MLC3F; Myosin light chain alkali 1/2; Myosin light chain A1/A2; anti-MYL1 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This MYL1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 24-58 amino acids from the human region of human MYL1.
Purity/Purification
Purified through a protein A column, followed by peptide affinity purification.
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
194
Applicable Applications for anti-MYL1 antibody
Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA)
Application Notes
IHC-P: 1:25
WB: 1:2000
WB: 1:2000
Antigen Type
Synthesized Peptide
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-MYL1 Antibody (N-Term) at 1:2000 dilutionLane 1: Human skeletal muslce lysateLane 2: Rat skeletal muscle lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-MYL1 Antibody (N-Term) at 1:2000 dilutionLane 1: Human skeletal muslce lysateLane 2: Rat skeletal muscle lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-MYL1 antibody
Regulatory light chain of myosin. Does not bind calcium.
Product Categories/Family for anti-MYL1 antibody
References
Seidel U.,et al.Nucleic Acids Res. 15:4989-4989(1987). Seidel U.,et al.Gene 66:135-146(1988). Ebert L.,et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004). Mural R.J.,et al. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21145
NCBI Official Full Name
myosin light chain 1/3, skeletal muscle isoform isoform 1f
NCBI Official Synonym Full Names
myosin light chain 1
NCBI Official Symbol
MYL1
NCBI Official Synonym Symbols
MLC1F; MLC3F
NCBI Protein Information
myosin light chain 1/3, skeletal muscle isoform
UniProt Protein Name
Myosin light chain 1/3, skeletal muscle isoform
Protein Family
UniProt Gene Name
MYL1
UniProt Synonym Gene Names
MLC1/MLC3; MLC1F/MLC3F; Myosin light chain A1/A2
NCBI Description
Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Regulatory light chain of myosin. Does not bind calcium.
Research Articles on MYL1
Similar Products
Product Notes
The MYL1 myl1 (Catalog #AAA9231261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYL1 Antibody (N-Term) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MYL1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA). IHC-P: 1:25 WB: 1:2000. Researchers should empirically determine the suitability of the MYL1 myl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
