Western Blot (WB)
(Western blot analysis of UCH-L5 expression in HT29 (A), Hela (B), mouse liver (C), rat brain (D) whole cell lysates.)
Rabbit UCH-L5 Polyclonal Antibody | anti-UCHL5 antibody
Anti-UCH-L5 Antibody
Western Blot (WB)
(Western blot analysis of UCH-L5 expression in HT29 (A), Hela (B), mouse liver (C), rat brain (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of UCH-L5 expression in HT29 (A), Hela (B), mouse liver (C), rat brain (D) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of UCH-L5 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of UCH-L5 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
Uniprot Description
UCHL5: Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1. Belongs to the peptidase C12 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.19.12; Ubiquitin conjugating system; Ubiquitin-specific protease; Protease
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: cytosol; nucleus; proteasome complex
Molecular Function: endopeptidase inhibitor activity; protein binding; ubiquitin-specific protease activity
Biological Process: DNA recombination; DNA repair; forebrain morphogenesis; lateral ventricle development; midbrain development; protein deubiquitination; regulation of transcription, DNA-dependent; transcription, DNA-dependent; ubiquitin-dependent protein catabolic process
Research Articles on UCHL5
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Product Notes
The UCHL5 uchl5 (Catalog #AAA8245686) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-UCH-L5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's UCH-L5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/ICC: 1/50 - 1/100. Researchers should empirically determine the suitability of the UCHL5 uchl5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UCH-L5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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