Rabbit UBE4A Polyclonal Antibody | anti-UBE4A antibody

UBE4A Polyclonal Antibody

Cat. Num. AAA3006280

• Gene Names: UBE4A; E4; UFD2; UBOX2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: UBE4A; Polyclonal Antibody; UBE4A Polyclonal Antibody; Ubiquitin conjugation factor E4 A; KIAA0126; anti-UBE4A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: UBE4A polyclonal antibody detects endogenous levels of UBE4A protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1066

• Applicable Applications for anti-UBE4A antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 183-228 of Human UBE4A.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of UBE4A polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-UBE4A antibody

UBE4A (ubiquitin conjugation factor E4 A), also known as E4, UFD2 or UBOX2, is a 1, 066 amino acid protein that functions in the multiubiquitin pathway of protein degradation. Expressed in cortical neurons and in tubular kidney cells, UBE4A is the human homolog of the Sacch-aromyces cerevisiae UFD2 protein and functions with the UBE (ubiquitin enzymes) proteins to catalyze ubiquitin chain assembly. UBE4A may be in-volved in cell growth and differentiation and can act as an autoantigen in scleroderma, a disease characterized by excessive deposits of collagen in the skin or other organs. Two isoforms of UBE4A exist due to alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 323362983
• NCBI GeneID: 9354
• NCBI Accession #: NP_001191006
• NCBI GenBank Nucleotide #: NP_001191006.1
• UniProt Accession #: Q14139
• Molecular Weight: ~ 125kDa
• NCBI Official Full Name: ubiquitin conjugation factor E4 A isoform 2
• NCBI Official Synonym Full Names: ubiquitination factor E4A
• NCBI Official Symbol: UBE4A
• NCBI Official Synonym Symbols: E4; UFD2; UBOX2
• NCBI Protein Information: ubiquitin conjugation factor E4 A
• UniProt Protein Name: Ubiquitin conjugation factor E4 A
• Protein Family: Ubiquitin conjugation factor
• UniProt Gene Name: UBE4A
• UniProt Entry Name: UBE4A_HUMAN

NCBI Description

This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011]