Rabbit anti-Human UBE4A Polyclonal Antibody | anti-UBE4A antibody

UBE4A, CT (Ubiquitin Conjugation Factor E4 A, KIAA0126)

Cat. Num. AAA630821

• Gene Names: UBE4A; E4; UFD2; UBOX2; KIAA0126; MGC133315
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: UBE4A; Polyclonal Antibody; CT (Ubiquitin Conjugation Factor E4 A; KIAA0126); Anti -UBE4A; anti-UBE4A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UBE4A.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-UBE4A antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:200; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human UBE4A (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-UBE4A Antibody (C-term) in NCI-H460 and CEM cell line lysates (35ug/lane). UBE4A (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-UBE4A antibody

Ubiquitination requires a ubiquitin-activating enzyme, a ubiquitin-conjugating enzyme, and usually a substrate-specific ubiquitin-protein ligase. Multiubiquitination needs a fourth factor, to bind to the ubiquitin moieties of preformed conjugates and catalyze ubiquitin chain assembly in conjunction with the first three proteins.1 Human protein KIAA0126, with a predicted 1,073aa residues, is a member of the novel protein family defined by ubiquitination conjugation factor E4.2 In yeast, E4 activity is linked to cell survival under stress conditions, indicating that eucaryotes use E4-dependent proteolysis pathways for multiple cellular functions.

Product Categories/Family for anti-UBE4A antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 323362983
• NCBI GeneID: 9354
• NCBI Accession #: NP_001191006.1
• NCBI GenBank Nucleotide #: NM_001204077.1
• UniProt Accession #: Q14139; Q2M1H0; Q6P5T4; Q7Z639; B0YJB6; Q05BZ7
• Molecular Weight: 122,561 Da
• NCBI Official Full Name: ubiquitin conjugation factor E4 A isoform 2
• NCBI Official Synonym Full Names: ubiquitination factor E4A
• NCBI Official Symbol: UBE4A
• NCBI Official Synonym Symbols: E4; UFD2; UBOX2; KIAA0126; MGC133315
• NCBI Protein Information: ubiquitin conjugation factor E4 A; OTTHUMP00000237916; OTTHUMP00000237917; ubiquitination factor E4A (UFD2 homolog, yeast)
• UniProt Protein Name: Ubiquitin conjugation factor E4 A
• Protein Family: Ubiquitin conjugation factor
• UniProt Gene Name: UBE4A
• UniProt Synonym Gene Names: KIAA0126
• UniProt Entry Name: UBE4A_HUMAN

NCBI Description

This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq]

Uniprot Description

UBE4A: Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3. Belongs to the ubiquitin conjugation factor E4 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: cytoplasm; ubiquitin ligase complex

Molecular Function: protein binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; protein polyubiquitination