Rabbit anti-Human, Mouse CCNF Polyclonal Antibody | anti-CCNF antibody

CCNF, ID (CCNF, FBX1, FBXO1, Cyclin-F, F-box only protein 1) (FITC)

Cat. Num. AAA6282391

• Gene Names: CCNF; FBX1; FBXO1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CCNF; Polyclonal Antibody; ID (CCNF; FBX1; FBXO1; Cyclin-F; F-box only protein 1) (FITC); F-box only protein 1; anti-CCNF antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CCNF. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CCNF antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 496-524 in the central region of human CCNF, UniProt Accession #P41002.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS6009767. This demonstrates that MBS6009767 detected the CCNF protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse liver tissue lysates (35ug/lane) usingMBS6009767. This demonstrates that MBS6009767 detected the CCNF protein (arrow).)

Product Categories/Family for anti-CCNF antibody

Antibodies; Cyclin

References

1. D'Angiolella, V., et al. Nature 466(7302):138-142(2010). 2. Fung, T.K., et al. J. Biol. Chem. 277(38):35140-35149(2002). 3. Kong, M., et al. EMBO J. 19(6):1378-1388(2000). 4. Bai, C., et al. Cell 86(2):263-274(1996). 5. Bai, C., et al. EMBO J. 13(24):6087-6098(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 118572588
• NCBI GeneID: 899
• NCBI Accession #: NP_001752.2
• NCBI GenBank Nucleotide #: NM_001761.2
• UniProt Accession #: P41002; Q96EG9; B2R8H3
• Molecular Weight: 87,640 Da
• NCBI Official Full Name: cyclin-F
• NCBI Official Synonym Full Names: cyclin F
• NCBI Official Symbol: CCNF
• NCBI Official Synonym Symbols: FBX1; FBXO1
• NCBI Protein Information: cyclin-F; F-box only protein 1; G2/mitotic-specific cyclin-F
• UniProt Protein Name: Cyclin-F
• Protein Family: Cyclin
• UniProt Gene Name: CCNF
• UniProt Synonym Gene Names: FBX1; FBXO1
• UniProt Entry Name: CCNF_HUMAN

NCBI Description

This gene encodes a member of the cyclin family. Cyclins are important regulators of cell cycle transitions through their ability to bind and activate cyclin-dependent protein kinases. This member also belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it was one of the first proteins in which the F-box motif was identified. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNF: Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication. Belongs to the cyclin family. Cyclin AB subfamily.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: centriole; SCF ubiquitin ligase complex; nucleus

Molecular Function: protein binding

Biological Process: mitosis; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; re-entry into mitotic cell cycle; cell division; protein ubiquitination; placenta development