Rabbit anti-Human UBE3C Polyclonal Antibody | anti-UBE3C antibody

UBE3C antibody

Cat. Num. AAA9404647

• Gene Names: UBE3C; HECTH2
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: UBE3C; Polyclonal Antibody; UBE3C antibody; HECTH2; anti-UBE3C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total UBE3C antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1083

• Applicable Applications for anti-UBE3C antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human UBE3C.
• Target Name: UBE3C
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using UBE3C antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cell using UBE3C antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-UBE3C antibody

E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.

Product Categories/Family for anti-UBE3C antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 187960100
• NCBI GeneID: 9690
• NCBI Accession #: NP_055486.2
• NCBI GenBank Nucleotide #: NM_014671.2
• UniProt Accession #: Q15386; Q8TC15; Q96CR4; Q9UDU3; A4D235; A6NCP3
• Molecular Weight: 123kd
• NCBI Official Full Name: ubiquitin-protein ligase E3C
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3C
• NCBI Official Symbol: UBE3C
• NCBI Official Synonym Symbols: HECTH2
• NCBI Protein Information: ubiquitin-protein ligase E3C
• UniProt Protein Name: Ubiquitin-protein ligase E3C
• Protein Family: Ubiquitin-protein ligase
• UniProt Gene Name: UBE3C
• UniProt Synonym Gene Names: KIAA0010; KIAA10
• UniProt Entry Name: UBE3C_HUMAN

Uniprot Description

UBE3C: E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 7q36.3

Cellular Component: proteasome complex; cytoplasm; intracellular; nucleus

Molecular Function: protein binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process