Rabbit anti-Human UBE3C Polyclonal Antibody | anti-UBE3C antibody

UBE3C (Ubiquitin-protein Ligase E3C, HectH2, KIAA0010, KIAA10)

Cat. Num. AAA6000419

• Gene Names: UBE3C; HECTH2
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: UBE3C; Polyclonal Antibody; UBE3C (Ubiquitin-protein Ligase E3C; HectH2; KIAA0010; KIAA10); Anti -UBE3C (Ubiquitin-protein Ligase E3C; anti-UBE3C antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UBE3C.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MFSFEGDFKTRPKVSLGGASRKYSIQRSAFDRCATLSQSGGAFPIANGPNLTLLVRQLLFFYKQNEDSKRLIWLYQNLIKHSSLFVKQLDGSERLTCLFQIKRLMSLCCRLLQNCNDDSLNVALPMRMLEVFSSENTYLPVLQDASYVVSVIEQILHYMIHNGYYRSLYLLINSKLPSSIEYSDLSRVPIAKILLENVLKPLHFTYNSCPEGARQQVFTAFTEEFLAAPFTDQIFHFIIPALADAQTVFPYEPFLNALLLIESRCSRKSGGAPWLFYFVLTVGENYLGALSEEGLLVYLRVLQTFLSQLPVSPASASCHDSASDSEEESEEADKPSSPEDGRLSVSYITEECLKKLDTKQQTNTLLNLVWRDSASEEVFTTMASVCHTLMVQHRMMVPKVRVYK

• Applicable Applications for anti-UBE3C antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human UBE3C, aa1-404 (AAH26241.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of UBE3C expression in transfected 293T cell line by UBE3C polyclonal antibody. Lane 1: UBE3C transfected lysate (45.8kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-UBE3C antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 187960100
• NCBI GeneID: 9690
• NCBI Accession #: NP_055486.2
• NCBI GenBank Nucleotide #: NM_014671.2
• UniProt Accession #: Q15386; Q8TC15; Q96CR4; Q9UDU3; A4D235; A6NCP3
• Molecular Weight: 123,923 Da
• NCBI Official Full Name: ubiquitin-protein ligase E3C
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3C
• NCBI Official Symbol: UBE3C
• NCBI Official Synonym Symbols: HECTH2
• NCBI Protein Information: ubiquitin-protein ligase E3C; ubiquitin-protein isopeptide ligase (E3)
• UniProt Protein Name: Ubiquitin-protein ligase E3C
• Protein Family: Ubiquitin-protein ligase
• UniProt Gene Name: UBE3C
• UniProt Synonym Gene Names: KIAA0010; KIAA10
• UniProt Entry Name: UBE3C_HUMAN

Uniprot Description

UBE3C: E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 7q36.3

Cellular Component: proteasome complex; cytoplasm; intracellular; nucleus

Molecular Function: protein binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process