Western Blot (WB)
(Western blot analysis of UBE3B expression in HEK293T (A), Jurkat (B) whole cell lysates.)
Rabbit anti-Human UBE3B Polyclonal Antibody | anti-UBE3B antibody
Anti-UBE3B Antibody
Immunohistochemistry: (1/100 - 1/200)
Upon delivery aliquot and store at -20°C for one year.
Avoid freeze/ thaw cycles.
Western Blot (WB)
(Western blot analysis of UBE3B expression in HEK293T (A), Jurkat (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of UBE3B expression in HEK293T (A), Jurkat (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of UBE3B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of UBE3B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2012]
Uniprot Description
UBE3B: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 12q24.11
Molecular Function: ligase activity; ubiquitin-protein ligase activity
Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process
Disease: Kaufman Oculocerebrofacial Syndrome
Research Articles on UBE3B
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Product Notes
The UBE3B ube3b (Catalog #AAA8242163) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-UBE3B Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBE3B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000) Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the UBE3B ube3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE3B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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