Rabbit UBE2T Polyclonal Antibody | anti-UBE2T antibody

UBE2T Polyclonal Antibody

Cat. Num. AAA3007595

• Gene Names: UBE2T; FANCT; PIG50; HSPC150
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: UBE2T; Polyclonal Antibody; UBE2T Polyclonal Antibody; Ubiquitin-conjugating enzyme E2 T; Cell proliferation-inducing gene 50 protein; E2 ubiquitin-conjugating enzyme T; Ubiquitin carrier protein T; Ubiquitin-protein ligase T; HSPC150; PIG50; anti-UBE2T antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: UBE2T polyclonal antibody detects endogenous levels of UBE2T protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 197

• Applicable Applications for anti-UBE2T antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human UBE2T.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of UBE2T polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:H9C2 whole cell lysate (40ug))

Related Product Information for anti-UBE2T antibody

Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an epsilon-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3.Ubiquitin conjugating-enzyme 2T (UBE2T) is an E2 family member responsible for the ATP-dependent ubiquitin tagging of target proteins for degradation. Research studies indicate that UBE2T plays an important role in the Fanconi anemia pathway and that UBE2T expression is required for normal DNA repair through this pathway. Interaction between UBE2T and FANCL appears to stimulate UBE2T auto monoubiquitination, leading to UBE2T inactivation and negative regulation of the Fanconi anemia pathway. Additional research details upregulation of UBE2T expression in breast cancer cells and certain lung carcinomas, suggesting a possible involvement in these malignancies.

NCBI and Uniprot Product Information

• NCBI GI #: 7661808
• NCBI GeneID: 29089
• NCBI Accession #: NP_054895.1
• NCBI GenBank Nucleotide #: NP_054895.1
• UniProt Accession #: Q9NPD8
• Molecular Weight: ~ 25kDa
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 T isoform 1
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 T
• NCBI Official Symbol: UBE2T
• NCBI Official Synonym Symbols: FANCT; PIG50; HSPC150
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 T
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 T
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2T
• UniProt Entry Name: UBE2T_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the covalent attachment of ubiquitin to protein substrates. Defects in this gene have been associated with Fanconi anemia of complementation group T. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]

Uniprot Description

UBE2T: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; chromatin binding; ATP binding; ligase activity

Biological Process: protein monoubiquitination; protein autoubiquitination; DNA repair; response to DNA damage stimulus

Disease: Fanconi Anemia, Complementation Group T