Rabbit UBE2L3 Polyclonal Antibody | anti-UBE2L3 antibody

UBE2L3 Antibody (C-term)

Cat. Num. AAA9232036

• Gene Names: UBE2L3; E2-F1; L-UBC; UBCH7; UbcM4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Synonyms: UBE2L3; Polyclonal Antibody; UBE2L3 Antibody (C-term); Ubiquitin-conjugating enzyme E2 L3; L-UBC; UbcH7; Ubiquitin carrier protein L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin-protein ligase L3; UBCE7; UBCH7; anti-UBE2L3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This UBE2L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-153 amino acids from the C-terminal region of human UBE2L3.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Length: 154

• Applicable Applications for anti-UBE2L3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: WB: 1:1000; IHC-P: 1:50-100
• Antigen Source: Human
• Antigen Region: 123-153
• Antigen Type: Synthetic Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-UBE2L3 Antibody (C137) at 1:1000 dilutionLane 1: mouse testis lysatesLane 2: rat testis lysatesLane 3: human testis lysatesLane 4: U87-MG whole cell lysatesLane 5: K562 whole cell lysates Lane 6: PC-12 whole cell lysates Lane 7: NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 18 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)-Paraffin

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-UBE2L3 antibody

Description: Purified Rabbit Polyclonal Antibody (Pab)

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro.

References

Moynihan, T.P., et al., Genomics 51(1):124-127 (1998). Moynihan, T.P., et al., Mamm. Genome 7(7):520-525 (1996). Nuber, U., et al., J. Biol. Chem. 271(5):2795-2800 (1996). Robinson, P.A., et al., Mamm. Genome 6(10):725-731 (1995). Ardley, H.C., et al., Biochim. Biophys. Acta 1491 (1-3), 57-64 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 54039805
• NCBI GeneID: 7332
• UniProt Accession #: P68036; Q3MHP1; P68037
• Molecular Weight: 17862
• NCBI Official Full Name: Ubiquitin-conjugating enzyme E2 L3
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 L3
• NCBI Official Symbol: UBE2L3
• NCBI Official Synonym Symbols: E2-F1; L-UBC; UBCH7; UbcM4
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 L3
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 L3
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2L3
• UniProt Synonym Gene Names: UBCE7; UBCH7
• UniProt Entry Name: UB2L3_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

UBE2L3: Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down- regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. Interacts with PARK2; involved in ubiquitination and degradation of misfolded proteins. Interacts with UBE3A; used by the papilloma virus HPV-16 E6 protein to ubiquitinate p53/TP53. Interacts with CCNB1IP1, CBL, ZAP70, RNF19A, RNF19B and RNF144B. Interacts with ARIH1. Interacts with ARIH2 (via RING-type 1). Interacts with NCOA1; they functionally interact to regulate progesterone receptor transcriptional activity. May interact with NR3C1. Ubiquitous, with highest expression in testis. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Nuclear receptor co-regulator; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytoplasm; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; cell proliferation; protein polyubiquitination; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of protein ubiquitination; positive regulation of ubiquitin-protein ligase activity; protein modification process; protein ubiquitination