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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Ubiquitin-conjugating enzyme E2 L3 (UBE2L3) Standard Curve.)

Human Ubiquitin-Conjugating Enzyme E2 L3 (UBE2L3) ELISA Kit | UBE2L3 elisa kit

Human Ubiquitin-Conjugating Enzyme E2 L3 (UBE2L3) ELISA Kit

Gene Names
UBE2L3; E2-F1; L-UBC; UBCH7; UbcM4
Reactivity
Human
Synonyms
Ubiquitin-Conjugating Enzyme E2 L3 (UBE2L3); Human Ubiquitin-Conjugating Enzyme E2 L3 (UBE2L3) ELISA Kit; UBE2L3; E2-F1; L-UBC; UBCH7; UbcM4; ubiquitin carrier protein; ubiquitin-conjugating enzyme UBCH7; ubiquitin-protein ligase; UBE2L3 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Ubiquitin-conjugating enzyme E2 L3 (UBE2L3) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBE2L3. No significant cross-reactivity or interference between Human UBE2L3 and analogues was observed.
Sequence Length
154
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Ubiquitin-conjugating enzyme E2 L3 (UBE2L3) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Ubiquitin-conjugating enzyme E2 L3 (UBE2L3) Standard Curve.)
Related Product Information for UBE2L3 elisa kit
Description: This Human Ubiquitin-conjugating enzyme E2 L3 (UBE2L3) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2L3 in samples. An antibody specific for UBE2L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UBE2L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2L3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2L3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). Ubiquitin-conjugating enzyme E2 L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Ubiquitin-conjugating enzyme E2 L3
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 L3
NCBI Official Symbol
UBE2L3
NCBI Official Synonym Symbols
E2-F1; L-UBC; UBCH7; UbcM4
NCBI Protein Information
ubiquitin-conjugating enzyme E2 L3
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 L3
UniProt Gene Name
UBE2L3
UniProt Synonym Gene Names
UBCE7; UBCH7
UniProt Entry Name
UB2L3_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

UBE2L3: Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down- regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. Interacts with PARK2; involved in ubiquitination and degradation of misfolded proteins. Interacts with UBE3A; used by the papilloma virus HPV-16 E6 protein to ubiquitinate p53/TP53. Interacts with CCNB1IP1, CBL, ZAP70, RNF19A, RNF19B and RNF144B. Interacts with ARIH1. Interacts with ARIH2 (via RING-type 1). Interacts with NCOA1; they functionally interact to regulate progesterone receptor transcriptional activity. May interact with NR3C1. Ubiquitous, with highest expression in testis. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Nuclear receptor co-regulator; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytoplasm; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; cell proliferation; protein polyubiquitination; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of protein ubiquitination; positive regulation of ubiquitin-protein ligase activity; protein modification process; protein ubiquitination

Research Articles on UBE2L3

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Product Notes

The Human UBE2L3 ube2l3 (Catalog #AAA9713767) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713767 ELISA Kit recognizes Human UBE2L3. It is sometimes possible for the material contained within the vial of "Ubiquitin-Conjugating Enzyme E2 L3 (UBE2L3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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