Rabbit anti-Human, Mouse UBE2L3 Polyclonal Antibody | anti-UBE2L3 antibody

UBE2L3 Antibody

Cat. Num. AAA7105161

• Gene Names: UBE2L3; E2-F1; L-UBC; UBCH7; UbcM4
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G purified
• Synonyms: UBE2L3; Polyclonal Antibody; UBE2L3 Antibody; Ubiquitin-conjugating enzyme E2 L3; E2 ubiquitin-conjugating enzyme L3; L-UBC; UbcH7; Ubiquitin carrier protein L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin-protein ligase L3; UBCE7; UBCH7; anti-UBE2L3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 212

• Applicable Applications for anti-UBE2L3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200
• Immunogen: Recombinant Human Ubiquitin-conjugating enzyme E2 L3 protein (1-154AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Mouse heart tissueAll lanes: UBE2L3 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 18, 15, 25 KDaObserved band size: 18 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7105161 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7105161 at dilution of 1:100)

Related Product Information for anti-UBE2L3 antibody

Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.

Product Categories/Family for anti-UBE2L3 antibody

Epigenetics and Nuclear Signaling; Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 373432684
• NCBI GeneID: 7332
• NCBI Accession #: NP_001243284.1
• NCBI GenBank Nucleotide #: NM_001256355.1
• UniProt Accession #: P68036; P51966; P70653; Q9HAV1; B2R4A7; B4DDG1; B4DSZ4; E7EWS7
• Molecular Weight: 24,004 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 L3 isoform 4
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 L3
• NCBI Official Symbol: UBE2L3
• NCBI Official Synonym Symbols: E2-F1; L-UBC; UBCH7; UbcM4
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 L3
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 L3
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2L3
• UniProt Synonym Gene Names: UBCE7; UBCH7

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.