Rabbit UBE2G1 Polyclonal Antibody | anti-UBE2G1 antibody

UBE2G1 Polyclonal Antibody

Cat. Num. AAA3007382

• Gene Names: UBE2G1; UBC7; E217K; UBE2G
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: UBE2G1; Polyclonal Antibody; UBE2G1 Polyclonal Antibody; Ubiquitin-conjugating enzyme E2 G1; E2 ubiquitin-conjugating enzyme G1; E217K; UBC7; Ubiquitin carrier protein G1; Ubiquitin-protein ligase G1; UBE2G; anti-UBE2G1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: UBE2G1 polyclonal antibody detects endogenous levels of UBE2G1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 170

• Applicable Applications for anti-UBE2G1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 63-108 of Human UBE2G1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of UBE2G1 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:NIH-3T3 whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug))

Related Product Information for anti-UBE2G1 antibody

Ubiquitin is an abundant, highly conserved protein found in all eukaryotic cells either free or covalently attached to cellular proteins. The primary function of ubiquitin in mammalian systems is to clear abnormal, foreign, and improperly folded proteins by targeting them for proteosome degradation. In Saccharomyces cerevisiae, ubiquitin-like proteins include Rub1, Ula1, Uba3, Smt3, Ubc2, Ubc12 and Ubc9. Rub1 shares 53% homology with ubiquitin and requires activation via the E2 proteins, including Ula1, Uba3 and Ubc12 in order to conjugate to substrates directed to different proteolytic systems. Ubc4 catalyzes ubiquitination of IkBa in a phosphorylation and SCFB-TRCP dependent manner. In this particular reaction, E1 first transfers ubiquitin to the E2 component Ubc4, and Ubc4 then associates with E3 ligase, which conjugates the poly-ubiquitin chain on a target protein. In this fashion, the chain tags the IkBa for degradation by a proteasome thus lifting the inhibitory effect of IkBa on NFkB and allowing NFkB to enter the nucleus.

NCBI and Uniprot Product Information

• NCBI GI #: 13489085
• NCBI GeneID: 7326
• NCBI Accession #: NP_003333.1
• NCBI GenBank Nucleotide #: NP_003333.1
• UniProt Accession #: P62253
• Molecular Weight: ~ 19kDa
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 G1
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 G1
• NCBI Official Symbol: UBE2G1
• NCBI Official Synonym Symbols: UBC7; E217K; UBE2G
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 G1
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 G1
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2G1
• UniProt Synonym Gene Names: UBE2G
• UniProt Entry Name: UB2G1_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE2G1: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: cytoplasm

Molecular Function: ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: cellular protein catabolic process; ubiquitin-dependent protein catabolic process