Rabbit anti-Human UBE2D2 Polyclonal Antibody | anti-UBE2D2 antibody

UBE2D2, CT (UBE2D2, UBC4, UBC5B, UBCH4, UBCH5B, Ubiquitin-conjugating enzyme E2 D2, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2(17)KB 2, Ubiquitin-conjugating enzyme E2-17kD 2, Ubiquitin-protein ligase D2) (MaxLight 650)

Cat. Num. AAA6360637

• Gene Names: UBE2D2; UBC4; PUBC1; UBC4/5; UBCH5B; E2(17)KB2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: UBE2D2; Polyclonal Antibody; CT (UBE2D2; UBC4; UBC5B; UBCH4; UBCH5B; Ubiquitin-conjugating enzyme E2 D2; Ubiquitin carrier protein D2; Ubiquitin-conjugating enzyme E2(17)KB 2; Ubiquitin-conjugating enzyme E2-17kD 2; Ubiquitin-protein ligase D2) (MaxLight 650); Ubiquitin-protein ligase D2; anti-UBE2D2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-UBE2D2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: UBE2D2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human UBE2D2.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-UBE2D2 antibody

MaxLight650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor647, DyLight649, Cy5 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Product Categories/Family for anti-UBE2D2 antibody

Antibodies; Ubiquitin Ligases

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Wu, K., et al. Mol. Cell 37(6):784-796(2010). Vina-Vilaseca, A., et al. J. Biol. Chem. 285(10):7645-7656(2010). Sakata, E., et al. Structure 18(1):138-147(2010). Kamadurai, H.B., et al. Mol. Cell 36(6):1095-1102(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4507775
• NCBI GeneID: 7322
• NCBI Accession #: NP_003330.1; NP_862821.1
• NCBI GenBank Nucleotide #: NM_003339.2
• UniProt Accession #: P62837; P51669; Q3MN78; Q96RP6; D3DQC9
• Molecular Weight: 16,735 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 D2 isoform 1
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2D 2
• NCBI Official Symbol: UBE2D2
• NCBI Official Synonym Symbols: UBC4; PUBC1; UBC4/5; UBCH5B; E2(17)KB2
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 D2; ubiquitin-protein ligase D2; ubiquitin carrier protein D2; ubiquitin-conjugating enzyme E2-17 kDa 2; p53-regulated ubiquitin-conjugating enzyme 1; ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5)
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 D2
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2D2
• UniProt Synonym Gene Names: PUBC1; UBC4; UBC5B; UBCH4; UBCH5B
• UniProt Entry Name: UB2D2_HUMAN

NCBI Description

Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Uniprot Description

UBE2D2: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP- induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ubiquitin conjugating system; Ligase; Ubiquitin ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: nucleoplasm; protein complex; cytosol; ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; protein polyubiquitination; MyD88-independent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; protein modification process; protein ubiquitination; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway