Rabbit ARF1 Polyclonal Antibody | anti-ARF1 antibody

Anti-ARF1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103581

• Reactivity: Human; Predicted to React with: Mouse, Rat, Bovine, African clawed frog, Cynomolgus (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: ARF1; Polyclonal Antibody; Anti-ARF1 Antibody; Rabbit Polyclonal; ARF1 Antibody; Rabbit PAb; Antigen Affinity Purified; ADP ribosylation factor 1; anti-ARF1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat, Bovine, African clawed frog, Cynomolgus (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ARF1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ARF1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human ARF1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human ARF1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ARF1 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:19 kDa)

Related Product Information for anti-ARF1 antibody

Invasion of tumor cells is a key step in metastasis that depends largely on the ability of these cells to degrade the extracellular matrix. The ARF1+ARF4 and ARF1+ARF3 pairs are both required for integrity of recycling endosomes but are involved in distinct transport pathways: the former pair regulates retrograde transport from endosomes to the TGN, whereas the latter is required for the transferrin recycling pathway from endosomes to the plasma membrane. The ADP-ribosylation factors (ARFs) 1 and 6 are small GTP-binding proteins, highly expressed and activated in several breast cancer cell lines and are associated with enhanced migration and invasiveness. ARF1 is a key role in invasion of breast cancer cells and suggest that targeting the ARF/Rho/MLC signaling axis might be a promising strategy to inhibit invasiveness and metastasis. ARF1 regulates cell migration and provide this GTPase as a target for the development of new therapeutics in triple negative breast cancer. ADP-ribosylation factor 1 (ARF1) is a crucial regulator in vesicle-mediated membrane trafficking and involved in the activation of signaling molecules. The aberrant MAPK signaling in prostate cancer is, at least in part, under the control of ARF1 and that, similar to Ras, ARF1 is a critical regulator in prostate cancer progression. ARF1 may represent a key molecular target for prostate cancer therapeutics and identification.

NCBI and Uniprot Product Information

• NCBI GI #: 51316985
• NCBI GeneID: 375
• UniProt Accession #: P84077; P10947; P32889
• Molecular Weight: 181
• NCBI Official Full Name: ADP-ribosylation factor 1
• NCBI Official Synonym Full Names: ADP-ribosylation factor 1
• NCBI Official Symbol: ARF1
• NCBI Protein Information: ADP-ribosylation factor 1
• UniProt Protein Name: ADP-ribosylation factor 1
• Protein Family: Auxin response factor
• UniProt Gene Name: ARF1
• UniProt Entry Name: ARF1_HUMAN

NCBI Description

ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ARF1: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein; G protein, monomeric, ARF; Motility/polarity/chemotaxis; G protein, monomeric

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: Golgi membrane; postsynaptic membrane; neuron projection; sarcomere; focal adhesion; perinuclear region of cytoplasm; postsynaptic density; plasma membrane; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; receptor signaling protein activity

Biological Process: protein transport; cellular copper ion homeostasis; COPI coating of Golgi vesicle; viral reproduction; regulation of receptor internalization; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidylinositol biosynthetic process; retrograde vesicle-mediated transport, Golgi to ER; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; regulation of defense response to virus by virus