Goat UBC3B Polyclonal Antibody | anti-UBE2R2 antibody

Goat anti-UBC3B Antibody

Cat. Num. AAA422841

• Gene Names: UBE2R2; UBC3B; CDC34B; E2-CDC34B
• Reactivity: Tested: Human, Rat; Expected from sequence similarity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: UBC3B; Polyclonal Antibody; Goat anti-UBC3B Antibody; UBE2R2; CDC34B; FLJ20419; MGC10481; ubiquitin-conjugating enzyme E2R 2; ubiquitin-conjugating enzyme UBC3B; UBE2R2 antibody; UBC3B antibody; CDC34B antibody; FLJ20419 antibody; MGC10481 antibody; ubiquitin-conjugating enzyme E2R 2 antibody; ubiquitin-conjugating enzyme UBC3B antibody; anti-UBE2R2 antibody

• Host: Goat
• Reactivity: Tested: Human, Rat; Expected from sequence similarity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: AQQQMTSSQKALM
• Sequence Length: 238

• Applicable Applications for anti-UBE2R2 antibody: Peptide ELISA (EIA), Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 4000.; Immunohistochemistry: In paraffin embedded Human Testis shows textured nuclear staining of the primary spermatocytes. Recommended concentration, 2-4ug/ml.; Western Blot: No signal obtained yet but low background observed in Human Testis and U937 lysates at up to 1ug/ml.; Immunoelectron microscopy: This product stained chromatoid bodies in Rat spermatids.
• Immunogen: Peptide with sequence AQQQMTSSQKALM-C, from the N Terminus of the protein sequence according to NP_060281.
• Epitope: N Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((2ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 22212943
• NCBI GeneID: 54926
• NCBI Accession #: NP_060281
• NCBI GenBank Nucleotide #: NM_017811.3
• Molecular Weight: 27,166 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 R2
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 R2
• NCBI Official Symbol: UBE2R2
• NCBI Official Synonym Symbols: UBC3B; CDC34B; E2-CDC34B
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 R2
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 R2
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2R2
• UniProt Synonym Gene Names: CDC34B; UBC3B
• UniProt Entry Name: UB2R2_HUMAN

NCBI Description

Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation. [provided by RefSeq, Jul 2008]

Uniprot Description

UBC3B: an E2 ubiquitin conjugating enzyme similar to UBC3/CDC34. Phosphorylation of UBC3B by CK2 induces its interaction with beta-TrCP, regulating beta-TrCP substrate recognition and enhancing beta-catenin degradation.

Protein type: Ubiquitin conjugating system; Ubiquitin ligase; Ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: cytoplasm

Molecular Function: ATP binding; protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein monoubiquitination