Rabbit anti-Human, Mouse UBC13 Polyclonal Antibody | anti-UBC13 antibody

Anti-ubc13 Antibody

Cat. Num. AAA7607742

• Gene Names: UBE2N; UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen affinity purified
• Synonyms: UBC13; Polyclonal Antibody; Anti-ubc13 Antibody; BLU; UbcH ben; UBE2N; Ubiquitin carrier protein N; Ubiquitin protein ligase N; anti-UBC13 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen affinity purified
• Form/Format: Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Sequence Length: 152

• Applicable Applications for anti-UBC13 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:500
• Immunogen: Ubiquitin-conjugating enzyme E2 N
• Preparation and Storage: Store at -20 degree C for 24 months. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse heart tissue slide using MBS7607742 (ubc13 antibody) at dilution of 1:200)

Western Blot (WB)

(Rat heart tissue were subjected to SDS PAGE followed by western blot with MBS7607742 (ubc13 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 4507793
• NCBI GeneID: 7334
• NCBI Accession #: NP_003339.1
• NCBI GenBank Nucleotide #: NP_003339.1
• UniProt Accession #: P61088
• Molecular Weight: Observed MW: 17kDa
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 N
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 N
• NCBI Official Symbol: UBE2N
• NCBI Official Synonym Symbols: UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 N
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 N
• UniProt Gene Name: UBE2N
• UniProt Synonym Gene Names: BLU
• UniProt Entry Name: UBE2N_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008]

Uniprot Description

UBE2N: The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Heterodimer with UBE2V2. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with RNF8 and RNF168. Interacts with RNF11. Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the 'Lys-63'-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair. Interacts with ARIH2 (via RING-type 2). Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ligase; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; DNA repair, damage

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: protein complex; cytoplasm; nucleus; cytosol; ubiquitin ligase complex; UBC13-MMS2 complex

Molecular Function: ubiquitin binding; protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; regulation of histone ubiquitination; positive regulation of DNA repair; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of histone modification; postreplication repair; cytokine and chemokine mediated signaling pathway; DNA double-strand break processing; protein modification process; protein ubiquitination; proteolysis; T cell receptor signaling pathway; double-strand break repair via homologous recombination; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of DNA repair; toll-like receptor 5 signaling pathway; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway