Rabbit CNOT7 Polyclonal Antibody | anti-CNOT7 antibody

CNOT7 Polyclonal Antibody

Cat. Num. AAA3007479

• Gene Names: CNOT7; CAF1; CAF-1; Caf1a; hCAF-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: CNOT7; Polyclonal Antibody; CNOT7 Polyclonal Antibody; CCR4-NOT transcription complex subunit 7; BTG1-binding factor 1; CCR4-associated factor 1; CAF-1; Caf1a; anti-CNOT7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CNOT7 polyclonal antibody detects endogenous levels of CNOT7 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 285

• Applicable Applications for anti-CNOT7 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 10-54 of Human CNOT7
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CNOT7 polyclonal antibody at 1:500 dilutionLane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:DLD whole cell lysate (40ug) Lane4:The Brain tissue lysate of Rat (40ug) Lane5:The Brain tissue lysate of Mouse (40ug))

Related Product Information for anti-CNOT7 antibody

CNOT7 (CCR4-NOT transcription complex, subunit 7), also known as CAF1 (CCR4-associated factor 1), hCAF-1 or BTG1-binding factor 1, is a member of the CAF1 family. Localizing to the nucleus, CNOT7 is ubiquitously expressed and is believed to function as a transcription factor, playing a role in a wide variety of processes. CNOT7 functions as a component of the evolutionarily conserved CCR4-NOT complex, a multi-subunit complex that participates in transcription as well as mRNA degradation. CNOT7 and other subunits of the CCR4-NOT complex play a role in the regulation of nuclear hormone receptor activities. CNOT7 directly binds to and interacts with RXR?, TOB1, TOB2, BTG1, BTG2 and BTG3. In addition, CNOT7 knockout mice are sterile and show an increase in bone mass, suggesting an important role for CNOT7 in spermatogenesis and as a suppressor of bone mass and BMP (bone morphogenetic protein) actions in osteoblasts.

NCBI and Uniprot Product Information

• NCBI GI #: 85067507
• NCBI GeneID: 29883
• NCBI Accession #: NP_037486.2
• NCBI GenBank Nucleotide #: NP_037486.2
• UniProt Accession #: Q9UIV1
• Molecular Weight: ~ 32kDa
• NCBI Official Full Name: CCR4-NOT transcription complex subunit 7 isoform 1
• NCBI Official Synonym Full Names: CCR4-NOT transcription complex subunit 7
• NCBI Official Symbol: CNOT7
• NCBI Official Synonym Symbols: CAF1; CAF-1; Caf1a; hCAF-1
• NCBI Protein Information: CCR4-NOT transcription complex subunit 7
• UniProt Protein Name: CCR4-NOT transcription complex subunit 7
• Protein Family: CCR4-NOT transcription complex
• UniProt Gene Name: CNOT7
• UniProt Synonym Gene Names: CAF1; CAF-1
• UniProt Entry Name: CNOT7_HUMAN

NCBI Description

The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The encoded protein downregulates the innate immune response and therefore provides a therapeutic target for enhancing its antimicrobial activity against foreign agents. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq, Apr 2016]

Uniprot Description

CNOT7: Ubiquitous transcription factor required for a diverse set of processes. It is a component of the CCR4 complex involved in the control of gene expression. Belongs to the CAF1 family.

Protein type: EC 3.1.13.4; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 8p22-p21.3

Cellular Component: membrane; cytosol; nucleus; CCR4-NOT complex

Molecular Function: signal transducer activity; protein binding; 3'-5'-exoribonuclease activity; poly(A)-specific ribonuclease activity; exoribonuclease activity; RNA binding; metal ion binding; transcription factor activity

Biological Process: deadenylation-dependent decapping; transcription, DNA-dependent; signal transduction; miRNA-mediated gene silencing; regulation of translation; negative regulation of cell proliferation; poly(A) tail shortening; positive regulation of cell proliferation; carbohydrate metabolic process; RNA-mediated gene silencing; positive regulation of transcription from RNA polymerase II promoter; gene expression; cytoplasmic mRNA processing body assembly; mRNA catabolic process, deadenylation-dependent decay