Rabbit anti-Human UBA7 Polyclonal Antibody | anti-UBA7 antibody

UBA7, NT (UBA7, UBE1L, UBE2, Ubiquitin-like modifier-activating enzyme 7, D8, Ubiquitin-activating enzyme E1 homolog)

Cat. Num. AAA647189

• Gene Names: UBA7; D8; UBE2; UBA1B; UBE1L
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: UBA7; Polyclonal Antibody; NT (UBA7; UBE1L; UBE2; Ubiquitin-like modifier-activating enzyme 7; D8; Ubiquitin-activating enzyme E1 homolog); Anti -UBA7; anti-UBA7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-UBA7 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: UBA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-268 amino acids from the N-terminal region of human UBA7.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(UBA7 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the UBA7 antibody detected the UBA7 protein (arrow).)

Immunohistochemistry (IHC)

(UBA7 Antibody (N-term) (AP14193a)immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBA7 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-UBA7 antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq].

Product Categories/Family for anti-UBA7 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 38045948
• NCBI GeneID: 7318
• NCBI Accession #: NP_003326.2
• NCBI GenBank Nucleotide #: NM_003335.2
• UniProt Accession #: P41226; Q9BRB2
• Molecular Weight: 111,694 Da
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme 7
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 7
• NCBI Official Symbol: UBA7
• NCBI Official Synonym Symbols: D8; UBE2; UBA1B; UBE1L
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme 7; ubiquitin-activating enzyme 7; ubiquitin-activating enzyme-2; UBA7, ubiquitin-activating enzyme E1; ubiquitin-activating enzyme E1 homolog; UBA1, ubiquitin-activating enzyme E1 homolog B; ubiquitin-activating enzyme E1-related protein
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme 7
• Protein Family: Ubiquitin-like modifier-activating enzyme
• UniProt Gene Name: UBA7
• UniProt Synonym Gene Names: UBE1L; UBE2; Ubiquitin-activating enzyme 7
• UniProt Entry Name: UBA7_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein. Ref.8 Ref.9

Pathway: Protein modification; protein ubiquitination. Ref.7

Subunit structure: Monomer

By similarity. Binds and is involved in the conjugation of G1P2/ISG15.

Tissue specificity: Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.

Post-translational modification: ISGylated. Ref.10

Miscellaneous: There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.

Sequence similarities: Belongs to the ubiquitin-activating E1 family.