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Western Blot (WB) (Western BlotPositive WB detected in: HepG2 whole cell lysate,Jurkat whole cell lysate,A549 whole cell lysateAll lanes: UAP1 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 59,58 KDaObserved band size: 59 KDa)

Rabbit anti-Human UAP1 Polyclonal Antibody | anti-UAP1 antibody

UAP1 Antibody

Gene Names
UAP1; AGX; AGX1; AGX2; SPAG2
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
UAP1; Polyclonal Antibody; UAP1 Antibody; UDP-N-acetylhexosamine pyrophosphorylase; Antigen X; AGX; Sperm-associated antigen 2; UDP-N-acetylgalactosamine pyrophosphorylase; AGX-1; UDP-N-acetylglucosamine pyrophosphorylase; AGX-2; SPAG2; anti-UAP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
505
Applicable Applications for anti-UAP1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1: 500-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200
Immunogen
Recombinant Human UDP-N-acetylhexosamine pyrophosphorylase protein (1-522AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Conjugation
Non-conjugated
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: HepG2 whole cell lysate,Jurkat whole cell lysate,A549 whole cell lysateAll lanes: UAP1 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 59,58 KDaObserved band size: 59 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: HepG2 whole cell lysate,Jurkat whole cell lysate,A549 whole cell lysateAll lanes: UAP1 antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 59,58 KDaObserved band size: 59 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7050172 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7050172 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of A549 cells using MBS7050172 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunohistochemistry (IHC) (Immunofluorescent analysis of A549 cells using MBS7050172 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-UAP1 antibody
Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
58,682 Da
NCBI Official Full Name
UDP-N-acetylhexosamine pyrophosphorylase isoform a
NCBI Official Synonym Full Names
UDP-N-acetylglucosamine pyrophosphorylase 1
NCBI Official Symbol
UAP1
NCBI Official Synonym Symbols
AGX; AGX1; AGX2; SPAG2
NCBI Protein Information
UDP-N-acetylhexosamine pyrophosphorylase
UniProt Protein Name
UDP-N-acetylhexosamine pyrophosphorylase
UniProt Gene Name
UAP1
UniProt Synonym Gene Names
SPAG2; AGX
UniProt Entry Name
UAP1_HUMAN

Uniprot Description

UAP1: Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P. Belongs to the UDPGP type 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 2.7.7.23; EC 2.7.7.83; Motility/polarity/chemotaxis; Transferase

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: cytoplasm; cytosol; nucleoplasm; plasma membrane

Molecular Function: identical protein binding; UDP-N-acetylglucosamine diphosphorylase activity

Biological Process: UDP-N-acetylglucosamine biosynthetic process

Research Articles on UAP1

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Product Notes

The UAP1 uap1 (Catalog #AAA7050172) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UAP1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UAP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1: 500-1: 5000 IHC: 1: 20-1: 200 IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the UAP1 uap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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