Rabbit anti-Human TYROBP Polyclonal Antibody | anti-TYROBP antibody

TYROBP, CT (TYROBP, DAP12, KARAP, TYRO protein tyrosine kinase-binding protein, DNAX-activation protein 12, Killer-activating receptor-associated protein) (MaxLight 750)

Cat. Num. AAA6360451

• Gene Names: TYROBP; DAP12; KARAP; PLOSL
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: TYROBP; Polyclonal Antibody; CT (TYROBP; DAP12; KARAP; TYRO protein tyrosine kinase-binding protein; DNAX-activation protein 12; Killer-activating receptor-associated protein) (MaxLight 750); Killer-activating receptor-associated protein; anti-TYROBP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TYROBP.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-TYROBP antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 85-113 in the C-terminal region of human TYROBP. Species sequence homology: bovine
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) using MBS642647. TYROBP (arrow) was detected using MBS642647.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma using MBS642647 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HepG2 cells using MBS642647 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-TYROBP antibody

Antibodies; Protein Kinases

References

1. Chen, X., et al. Blood 113(14):3226-3234(2009). 2. Sulonen, A.M., et al. J. Neuroimmunol. 206 (1-2), 86-90 (2009). 3. Lanier, L.L. Immunol. Rev. 227(1):150-160(2009). 4. Schleinitz, N., et al. PLoS ONE 4 (7), E6264 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4507755
• NCBI GeneID: 7305
• NCBI Accession #: NP_003323.1
• NCBI GenBank Nucleotide #: NM_003332.3
• UniProt Accession #: O43914; Q6FGA5; Q9UMT3; A8K2X0; F5H389
• Molecular Weight: 11,043 Da
• NCBI Official Full Name: TYRO protein tyrosine kinase-binding protein isoform 1
• NCBI Official Synonym Full Names: TYRO protein tyrosine kinase binding protein
• NCBI Official Symbol: TYROBP
• NCBI Official Synonym Symbols: DAP12; KARAP; PLOSL
• NCBI Protein Information: TYRO protein tyrosine kinase-binding protein; KAR-associated protein; DNAX-activation protein 12; killer-activating receptor-associated protein
• UniProt Protein Name: TYRO protein tyrosine kinase-binding protein
• Protein Family: TYRO protein tyrosine kinase-binding protein
• UniProt Gene Name: TYROBP
• UniProt Synonym Gene Names: DAP12; KARAP; KAR-associated protein
• UniProt Entry Name: TYOBP_HUMAN

NCBI Description

This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Multiple alternative transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

TYROBP: Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin. Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. Belongs to the TYROBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: identical protein binding; protein binding; receptor signaling protein activity; receptor binding

Biological Process: integrin-mediated signaling pathway; axon guidance; regulation of immune response; neutrophil activation during immune response; macrophage activation during immune response; innate immune response; cellular defense response; signal transduction

Disease: Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy