Western Blot (WB)
(Western blot analysis of Tyrosinase Antibody (Center) in A375, 293 cell line lysates (35ug/lane). Tyrosinase (arrow) was detected using the purified Pab.)
Rabbit anti-Human TYR Polyclonal Antibody
TYR, ID (Tyrosinase, Monophenol Monooxygenase, Tumor Rejection Antigen AB, SK29-AB, LB24-AB)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
PurifiedPurified by ammonium sulfate precipitation
Synonyms
TYR; Polyclonal Antibody; ID (Tyrosinase; Monophenol Monooxygenase; Tumor Rejection Antigen AB; SK29-AB; LB24-AB); Anti -TYR; anti-TYR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Tyrosinase.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation
Purified by ammonium sulfate precipitation
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide
Applicable Applications for anti-TYR antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence.
Immunogen
Synthetic peptide corresponding to aa183-213 from the center region of human Tyrosinase conjugated to KLH. Cellular Localization: Melanosome membrane; single-pass, type 1 membranre protein
Cellular Localization
Melanosome membrane; single-pass, type 1 membranre protein
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of Tyrosinase Antibody (Center) in A375, 293 cell line lysates (35ug/lane). Tyrosinase (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of Tyrosinase Antibody (Center) in A375, 293 cell line lysates (35ug/lane). Tyrosinase (arrow) was detected using the purified Pab.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of TYR Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of TYR Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunohistochemistry (IHC)
(TYR Antibody (Center) (AP7345c)immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TYR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(TYR Antibody (Center) (AP7345c)immunohistochemistry analysis in formalin fixed and paraffin embedded human melanoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TYR Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-TYR antibody
TYR catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The protein has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this protein result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation.
Product Categories/Family for anti-TYR antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The TYR (Catalog #AAA627694) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TYR, ID (Tyrosinase, Monophenol Monooxygenase, Tumor Rejection Antigen AB, SK29-AB, LB24-AB) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TYR can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Researchers should empirically determine the suitability of the TYR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TYR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
