Rabbit anti-Human, Mouse TXNDC12 Polyclonal Antibody | anti-TXNDC12 antibody

TXNDC12, CT (TXNDC12, TLP19, Thioredoxin domain-containing protein 12, Endoplasmic reticulum resident protein 18, Endoplasmic reticulum resident protein 19, Thioredoxin-like protein p19, hTLP19) (PE)

Cat. Num. AAA6360342

• Gene Names: TXNDC12; AG1; AGR1; ERP16; ERP18; ERP19; TLP19; hAG-1; PDIA16; hTLP19
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TXNDC12; Polyclonal Antibody; CT (TXNDC12; TLP19; Thioredoxin domain-containing protein 12; Endoplasmic reticulum resident protein 18; Endoplasmic reticulum resident protein 19; Thioredoxin-like protein p19; hTLP19) (PE); hTLP19; anti-TXNDC12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TXNDC12 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TXNDC12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acids 140-170 from the C-terminal region of human TXNDC12.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TXNDC12 Antibody (C-term) in Y79 cell line lysates (35ug/lane). TXNDC12 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of TXNDC12 Antibody (C-term) in mouse stomach tissue lysates (35ug/lane). TXNDC12 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(TXNDC12 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TXNDC12 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-TXNDC12 antibody

TXNDC12 belongs to the thioredoxin superfamily (see TXN; MIM 187700). Members of this superfamily possess a thioredoxin fold with a consensus active-site sequence (CxxC) and have roles in redox regulation, defense against oxidative stress, refolding of disulfide-containing proteins, and regulation of transcription factors

Product Categories/Family for anti-TXNDC12 antibody

Antibodies; Thioredoxin

References

Jessop, C.E., et al. J. Cell. Sci. 122 (PT 23), 4287-4295 (2009). Rowe, M.L., et al. Biochemistry 48(21):4596-4606(2009). Jeong, W., et al. J. Biol. Chem. 283(37):25557-25566(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 7705696
• NCBI GeneID: 51060
• NCBI Accession #: NP_056997.1
• NCBI GenBank Nucleotide #: NM_015913.3
• UniProt Accession #: O95881; Q5T1T4; Q96H50; B3KQS0
• Molecular Weight: 19,206 Da
• NCBI Official Full Name: thioredoxin domain-containing protein 12
• NCBI Official Synonym Full Names: thioredoxin domain containing 12 (endoplasmic reticulum)
• NCBI Official Symbol: TXNDC12
• NCBI Official Synonym Symbols: AG1; AGR1; ERP16; ERP18; ERP19; TLP19; hAG-1; PDIA16; hTLP19
• NCBI Protein Information: thioredoxin domain-containing protein 12; ER protein 18; ER protein 19; anterior gradient homolog 1; endoplasmic reticulum protein ERp19; endoplasmic reticulum resident protein 18; endoplasmic reticulum resident protein 19; endoplasmic reticulum thioredox
• UniProt Protein Name: Thioredoxin domain-containing protein 12
• Protein Family: Thioredoxin domain-containing protein
• UniProt Gene Name: TXNDC12
• UniProt Synonym Gene Names: TLP19; ER protein 18; ERp18; ER protein 19; ERp19
• UniProt Entry Name: TXD12_HUMAN

NCBI Description

This gene encodes a member of the thioredoxin superfamily. Members of this family are characterized by a conserved active motif called the thioredoxin fold that catalyzes disulfide bond formation and isomerization. This protein localizes to the endoplasmic reticulum and has a single atypical active motif. The encoded protein is mainly involved in catalyzing native disulfide bond formation and displays activity similar to protein-disulfide isomerases. This protein may play a role in defense against endoplasmic reticulum stress. Alternate splicing results in both coding and non-coding variants. [provided by RefSeq, Mar 2012]

Uniprot Description

TXNDC12: Possesses significant protein thiol-disulfide oxidase activity.

Protein type: EC 1.8.4.2; Secreted; Other Amino Acids Metabolism - glutathione; Secreted, signal peptide; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: endoplasmic reticulum lumen

Molecular Function: protein-disulfide reductase (glutathione) activity

Biological Process: cell redox homeostasis