Rabbit TUSA Polyclonal Antibody | anti-TUSA antibody

Rabbit anti-Escherichia coli (strain K12) TUSA Polyclonal Antibody

• Gene Names: tusA; ECK3454; JW3435; sirA; yhhP
• Reactivity: E Coli (strain K12)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: TUSA; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) TUSA Polyclonal Antibody; TUSA Antibody; Sulfur carrier protein TusA; Sulfur mediator TusA; Sulfur transfer protein TusA; tRNA 2-thiouridine synthesizing protein A; tusA sirA yhhP b3470 JW3435; anti-TUSA antibody

• Host: Rabbit
• Reactivity: E Coli (strain K12)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-TUSA antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Escherichia coli (strain K12) tusA protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 16131342
• NCBI GeneID: 947974
• NCBI Accession #: NP_417927.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0A890; P37618; Q2M7D3
• Molecular Weight: 9,095 Da
• NCBI Official Full Name: mnm(5)-s(2)U34-tRNA 2-thiolation sulfurtransferase
• NCBI Official Symbol: tusA
• NCBI Official Synonym Symbols: ECK3454; JW3435; sirA; yhhP
• NCBI Protein Information: mnm(5)-s(2)U34-tRNA 2-thiolation sulfurtransferase
• UniProt Protein Name: Sulfur carrier protein TusA
• Protein Family: Sulfurtransferase
• UniProt Gene Name: tusA

NCBI Description

SirA(TusA) interacts with and activates IscS, accepting sulfur from IscS to form C19 persulfide; transfers C19 persulfide to C78 of YheN(TusD). [More information is available at EcoGene: EG12216]. TusA functions as a sulfur mediator during synthesis of 2-thiouridine of the modified wobble base mnm5s2U in tRNA. [More information is available at EcoCyc: EG12216].

Uniprot Description

Sulfur carrier protein involved in sulfur trafficking in the cell. Part of a sulfur-relay system required for 2-thiolation during synthesis of 2-thiouridine of the modified wobble base 5-methylaminomethyl-2-thiouridine (mnm5s2U) in tRNA (PubMed:16387657). Interacts with IscS and stimulates its cysteine desulfurase activity (PubMed:16387657, PubMed:23281480). Accepts an activated sulfur from IscS, which is then transferred to TusD, and thus determines the direction of sulfur flow from IscS to 2-thiouridine formation (PubMed:16387657). Also appears to be involved in sulfur transfer for the biosynthesis of molybdopterin (PubMed:23281480). Seems to affect the stability of sigma-S, particularly during the logarithmic growth phase (PubMed:9555915).