Rabbit anti-Human Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) Polyclonal Antibody | anti-ACAT1 antibody

APC/Cy7 Linked Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1)

Cat. Num. AAA2110029

• Gene Names: ACAT1; T2; MAT; ACAT; THIL
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Acetyl Coenzyme A Acetyltransferase 1 (ACAT1); Polyclonal Antibody; APC/Cy7 Linked Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1); THIL; ACAT; T2; MAT; Acetoacetyl Coenzyme A Thiolase; anti-ACAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a Rabbit polyclonal antibody raised against ACAT1. It has been selected for its ability to recognize ACAT1 in immunohistochemical staining and western blotting.
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Liquid
• Concentration: >=100ug/ml (varies by lot)
• Sequence: Ala111~Leu427
• Sequence Length: 427

• Applicable Applications for anti-ACAT1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Conjugation: APC-Cy7
• Cross Reactivity: Human
• Preparation and Storage: Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.; Avoid repeated freeze/thaw cycles.; ; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Knockout Validation

(Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: ACAT1 knockout A549 cell lysate; Predicted MW: 17,45kd Observed MW: 44kd Primary Ab: 1ug/ml Rabbit Anti-Human ACAT1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

NCBI and Uniprot Product Information

• NCBI GI #: 4557237
• NCBI GeneID: 38
• NCBI Accession #: NP_000010.1
• NCBI GenBank Nucleotide #: NM_000019.3
• UniProt Accession #: P24752
• NCBI Official Full Name: acetyl-CoA acetyltransferase, mitochondrial
• NCBI Official Synonym Full Names: acetyl-CoA acetyltransferase 1
• NCBI Official Symbol: ACAT1
• NCBI Official Synonym Symbols: T2; MAT; ACAT; THIL
• NCBI Protein Information: acetyl-CoA acetyltransferase, mitochondrial
• UniProt Protein Name: Acetyl-CoA acetyltransferase, mitochondrial
• UniProt Gene Name: ACAT1
• UniProt Synonym Gene Names: ACAT; MAT
• UniProt Entry Name: THIL_HUMAN

NCBI Description

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]

Uniprot Description

ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family.

Protein type: Mitochondrial; Lipid Metabolism - synthesis and degradation of ketone bodies; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - lysine degradation; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - butanoate; EC 2.3.1.9; Lipid Metabolism - fatty acid; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Acetyltransferase; Amino Acid Metabolism - tryptophan

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: protein homodimerization activity; enzyme binding; acetyl-CoA C-acetyltransferase activity; metal ion binding; coenzyme binding

Biological Process: response to starvation; ketone body catabolic process; response to hormone stimulus; ketone body biosynthetic process; branched chain family amino acid catabolic process; cellular lipid metabolic process; brain development; ketone body metabolic process; liver development; response to organic cyclic substance; protein homooligomerization

Disease: Alpha-methylacetoacetic Aciduria