Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Tubulin alpha-1B/K-alpha-1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Rabbit Tubulin alpha-1B/K-alpha-1 Polyclonal Antibody | anti-K-alpha-1 antibody
Tubulin alpha-1B/K-alpha-1 Rabbit pAb
IHC: 1:50-1:200
IF: 1:50-1:200
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Tubulin alpha-1B/K-alpha-1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Tubulin alpha-1B/K-alpha-1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Mouse spleen using Tubulin alpha-1B/K-alpha-1 antibody at dilution of 1:50 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Mouse spleen using Tubulin alpha-1B/K-alpha-1 antibody at dilution of 1:50 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Rat kidney using Tubulin alpha-1B/K-alpha-1 antibody at dilution of 1:50 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Rat kidney using Tubulin alpha-1B/K-alpha-1 antibody at dilution of 1:50 (40x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using Tubulin alpha-1B/K-alpha-1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3 cells using Tubulin alpha-1B/K-alpha-1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of C6 cells using Tubulin alpha-1B/K-alpha-1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of C6 cells using Tubulin alpha-1B/K-alpha-1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using Tubulin alpha-1B/K-alpha-1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using Tubulin alpha-1B/K-alpha-1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
Uniprot Description
TUBA1B: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 12q13.12
Cellular Component: microtubule; cytoplasmic microtubule
Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; double-stranded RNA binding; structural molecule activity
Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell division; cytoskeleton-dependent intracellular transport; microtubule cytoskeleton organization and biogenesis; microtubule-based process
Research Articles on K-alpha-1
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Product Notes
The K-alpha-1 tuba1b (Catalog #AAA9143941) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Tubulin alpha-1B/K-alpha-1 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Tubulin alpha-1B/K-alpha-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the K-alpha-1 tuba1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHPE QLITGKEDAA NNYARGHYTI GKEIIDLVLD RIRKLADQCT GLQGFLVFHS FGGGTGSGFT SLLMERLSVD YGKKSKLEFS IYPAPQVSTA VVEPYNSILT THTTLEHSDC AFMVDNEAIY DICRRNLDIE RPTYTNLNRL ISQIVSSITA SLRFDGALNV DLTEFQTNLV PYPRIHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN QMVKCDPRHG KYMACCLLYR GDVVPKDVNA AIATIKTKRS IQFVDWCPTG FKVGINYQPP TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LDHKFDLMYA KRAFVHWYVG EGMEEGEFSE AREDMAALEK DYEEVGVDSV EGEGEEEGEE Y. It is sometimes possible for the material contained within the vial of "Tubulin alpha-1B/K-alpha-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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