Rabbit Tuberin/TSC2 Polyclonal Antibody | anti-TSC2 antibody

Phospho-Tuberin/TSC2 (Ser664) Antibody

Cat. Num. AAA9613238

• Gene Names: TSC2; LAM; TSC4
• Reactivity: Human; Predicted Reactivity: Pig (100%), Horse (100%)
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: Tuberin/TSC2; Polyclonal Antibody; Phospho-Tuberin/TSC2 (Ser664) Antibody; FLJ43106; LAM; OTTHUMP00000158940; OTTHUMP00000198394; OTTHUMP00000198395; PPP1R160; Protein phosphatase 1; regulatory subunit 160; tsc2; TSC2_HUMAN; TSC4; TSC4 gene; formerly; Tuberin; Tuberous sclerosis 2; Tuberous sclerosis 2 protein; Tuberous sclerosis 2 protein homolog; anti-TSC2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Pig (100%), Horse (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-Tuberin/TSC2 (Ser664) Antibody detects endogenous levels of Tuberin/TSC2 only when phosphorylated at Ser664.; Tissue Specificity: Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TSC2 antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Tuberin/TSC2 around the phosphorylation site of Ser664.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1. Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation. Phosphorylation by AMPK activates it and leads to negatively regulates the mTORC1 complex. Phosphorylated at Ser-1798 by RPS6KA1; phosphorylation inhibits TSC2 ability to suppress mTORC1 signaling. Phosphorylated by DAPK1.Ubiquitinated by the DCX (FBXW5) E3 ubiquitin-protein ligase complex, leading to its subsequent degradation. Ubiquitinated by MYCBP2 independently of its phosphorylation status leading to subsequent degradation; association with TSC1 protects from ubiquitination.
• Subunit Structure: Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones CDC37, PPP5C, TSC1 and client protein TSC2, CDK4, AKT, RAF1 and NR3C1; this complex does not contain co-chaperones STIP1/HOP and PTGES3/p23. Forms a complex containing HSP90AA1, TSC1 and TSC2; TSC1 is required to recruit TCS2 to the complex thereby stabilizing TSC2. Interacts with TSC1 and HERC1; the interaction with TSC1 stabilizes TSC2 and prevents the interaction with HERC1. May also interact with the adapter molecule RABEP1. The final complex may contain TSC2 and RABEP1 linked to RAB5. Interacts with HSPA1 and HSPA8. Interacts with DAPK1. Interacts with FBXW5. Interacts with NAA10 (via C-terminal domain). Interacts with RRAGA (polyubiquitinated). Interacts with WDR45B. Interacts with RPAP3 and URI1. (Microbial infection) Interacts with human cytomegalovirus protein UL38; this interaction inhibits cellular stress response mediated by mTORC1.
• Subcellular Location: Cytoplasm. Membrane>Peripheral membrane protein.; Note: At steady state found in association with membranes.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from 293 Anisomycin 25ug/ml 30', using Phospho-Tuberin/TSC2 (Ser664) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Related Product Information for anti-TSC2 antibody

In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. May also play a role in microtubule-mediated protein transport (By similarity). Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 116256352
• NCBI GeneID: 7249
• NCBI Accession #: NP_000539.2
• NCBI GenBank Nucleotide #: NM_000548.3
• UniProt Accession #: P49815; O75275; Q4LE71; Q8TAZ1; A7E2E2; B4DIQ7; B4DRN2; C9J378
• Molecular Weight: 200,608 Da
• NCBI Official Full Name: tuberin isoform 1
• NCBI Official Synonym Full Names: tuberous sclerosis 2
• NCBI Official Symbol: TSC2
• NCBI Official Synonym Symbols: LAM; TSC4
• NCBI Protein Information: tuberin; tuberous sclerosis 2 protein
• UniProt Protein Name: Tuberin
• Protein Family: Tuberin
• UniProt Gene Name: TSC2
• UniProt Synonym Gene Names: TSC4
• UniProt Entry Name: TSC2_HUMAN

NCBI Description

Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TSC2: a product of the tumor suppressor gene TSC2. Tuberin and Hamartin (TSC1) form a tumor suppressor heterodimer that inhibits the mTOR nutrient signaling input. TSC1/TSC2 targets the small G protein Rheb, a novel mediator of the nutrient signaling input to mTOR. Functions as a Rheb GTPase activating protein (GAP). Four alternatively spliced isoforms have been described.

Protein type: Tumor suppressor; GAPs, misc.; Cell cycle regulation; Nuclear receptor co-regulator; GAPs

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: TSC1-TSC2 complex; Golgi apparatus; cytoskeleton; growth cone; membrane; cell soma; perinuclear region of cytoplasm; cytoplasm; dendrite; caveola; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; GTPase activator activity; phosphatase binding

Biological Process: negative regulation of MAP kinase activity; phosphoinositide 3-kinase cascade; regulation of insulin receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; nerve growth factor receptor signaling pathway; protein heterooligomerization; regulation of cell cycle; heart development; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; negative regulation of cell proliferation; protein localization; positive chemotaxis; cell projection organization and biogenesis; protein import into nucleus; regulation of endocytosis; acute-phase response; protein kinase B signaling cascade; cell cycle arrest; protein homooligomerization; negative regulation of epithelial cell proliferation; epidermal growth factor receptor signaling pathway; negative regulation of TOR signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of protein kinase B signaling cascade; endocytosis; vesicle-mediated transport; insulin-like growth factor receptor signaling pathway; glucose import; neural tube closure; insulin receptor signaling pathway; response to hypoxia; innate immune response; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of phosphoinositide 3-kinase cascade

Disease: Lymphangioleiomyomatosis; Tuberous Sclerosis 2