Chicken anti-Mouse TUBB3 / Tubulin Beta 3 Polyclonal Antibody | anti-TUBB3 antibody

Anti-TUBB3 / Tubulin Beta 3 Antibody IHC-plus; Chicken Polyclonal (IgY) to Mouse TUBB3 / Tubulin Beta 3

Cat. Num. AAA244264

• Gene Names: TUBB3; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
• Reactivity: Mouse
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: TUBB3 / Tubulin Beta 3; Polyclonal Antibody; Anti-TUBB3 / Tubulin Beta 3 Antibody IHC-plus; Chicken Polyclonal (IgY) to Mouse TUBB3 / Tubulin Beta 3; TUBB3; CDCBM; Beta III Tubulin; Class III beta-tubulin; TUBB4; Tubulin; beta 3; Tubulin beta-III; Tubulin beta-3 chain; Tubulin beta-4 chain; beta 3 class III; CFEOM3A; anti-TUBB3 antibody

• Host: Chicken
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes beta-Tubulin 3. Species cross-reactivity: mouse. Species sequence homology: rat, human.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 10mM PBS, pH 7.2, 0.02% sodium azide.
• Concentration: 0.3 mg/ml (varies by lot)
• Sequence Length: 450

• Applicable Applications for anti-TUBB3 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: Uses: ICC (1:1000 - 1:2000); IHC-P (5 ug/ml); WB (1:2000 - 1:5000) Optimal dilutions to be determined by the researcher.; ; Usage Summary: Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Immunohistochemistry: 1:1000-1:2000. Immunocytochemistry: 1:1000-1:2000. Western Blot: 1:2000-1:5000.
• Target: Mouse TUBB3 / Tubulin Beta 3
• Immunogen Species: Antibody was raised against mouse
• Family/Subfamily: Tubulin
• Antigen Type: Synthetic peptide - KLH conjugated
• Immunogen: TUBB3 / Tubulin Beta 3 antibody was raised against three synthetic peptides corresponding to different regions of the beta-Tubulin 3 (KLH). Species sequence homology: human (NP_AAL28094, NCBI), rat (AAM28438, NCBI) 100%.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short-term: Store at 4 degree C.; Long-term: Aliquot and store at -20 degree C.; Avoid freeze-thaw cycles.; Aliquots are stable for 1 year after receipt.

Immunohistochemistry (IHC)

(Anti-Tuj1 antibody IHC of mouse brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-TUBB3 antibody

TUBB3 / Tubulin Beta 3 is a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants.

Product Categories/Family for anti-TUBB3 antibody

Family: Tubulin

NCBI and Uniprot Product Information

• NCBI GI #: 50592996
• NCBI GeneID: 10381
• NCBI Accession #: NP_006077.2
• NCBI GenBank Nucleotide #: NM_006086.3
• UniProt Accession #: Q13509; Q9BTZ0; Q9BW10; A8K854
• NCBI Official Full Name: tubulin beta-3 chain isoform 1
• NCBI Official Synonym Full Names: tubulin, beta 3 class III
• NCBI Official Symbol: TUBB3
• NCBI Official Synonym Symbols: CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A
• NCBI Protein Information: tubulin beta-3 chain; tubulin beta-III; tubulin beta-4 chain; class III beta-tubulin
• UniProt Protein Name: Tubulin beta-3 chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBB3
• UniProt Synonym Gene Names: TUBB4
• UniProt Entry Name: TBB3_HUMAN

NCBI Description

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Uniprot Description

TUBB3: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Dimer of alpha and beta chains. Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: microtubule; cell soma; axon; cytoplasm; dendrite; nucleus

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; peptide binding

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; mitosis; axon guidance; cellular protein metabolic process; protein folding; transmembrane transport; microtubule-based process

Disease: Fibrosis Of Extraocular Muscles, Congenital, 3a, With Or Without Extraocular Involvement; Cortical Dysplasia, Complex, With Other Brain Malformations 1