Rabbit TUBA4A Polyclonal Antibody | anti-TUBA4A antibody

TUBA4A (Tubulin alpha-4A chain, TUBA1, H2-Alpha) (FITC)

Cat. Num. AAA6441965

• Gene Names: TUBA4A; TUBA1; H2-ALPHA
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: TUBA4A; Polyclonal Antibody; TUBA4A (Tubulin alpha-4A chain; TUBA1; H2-Alpha) (FITC); anti-TUBA4A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes TUBA4A. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-TUBA4A antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:25-1:100; WB: 1:500-1:2000; IHC: 1:25-1:50; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human TUBA4A.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TUBA4A antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer tissue using TUBA4A antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using TUBA4A antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-TUBA4A antibody

Antibodies; Tubulin

NCBI and Uniprot Product Information

• NCBI GI #: 55977476
• NCBI GeneID: 7277
• UniProt Accession #: P68366; P05215; A8MUB1; B3KNQ6
• Molecular Weight: 448
• NCBI Official Full Name: Tubulin alpha-4A chain
• NCBI Official Synonym Full Names: tubulin, alpha 4a
• NCBI Official Symbol: TUBA4A
• NCBI Official Synonym Symbols: TUBA1; H2-ALPHA
• NCBI Protein Information: tubulin alpha-4A chain; tubulin H2-alpha; tubulin alpha-1 chain; tubulin, alpha 1 (testis specific)
• UniProt Protein Name: Tubulin alpha-4A chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBA4A
• UniProt Synonym Gene Names: TUBA1
• UniProt Entry Name: TBA4A_HUMAN

NCBI Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

TUBA4A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: microtubule; extracellular region; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; platelet activation; cellular protein metabolic process; platelet degranulation; protein folding; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; blood coagulation; microtubule-based process

Disease: Amyotrophic Lateral Sclerosis 22 With Or Without Frontotemporal Dementia