Rabbit TTN Polyclonal Antibody | anti-TTN antibody

TTN Polyclonal Antibody

Cat. Num. AAA9419862

• Gene Names: TTN; TMD; CMH9; CMD1G; CMPD4; EOMFC; HMERF; MYLK5; SALMY; LGMD2J
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
• Synonyms: TTN; Polyclonal Antibody; TTN Polyclonal Antibody; Titin (EC 2.7.11.1) (Connectin) (Rhabdomyosarcoma antigen MU-RMS-40.14); anti-TTN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous TTN
• Purity/Purification: Affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
• Form/Format: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-TTN antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-300
• Immunogen: Synthetic peptide from human protein at AA range: 161-210
• Immunogen Type: Protein
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human-heart, antibody was diluted at 1:200)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human-heart, antibody was diluted at 1:200)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human-skeletal-muscle, antibody was diluted at 1:200)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human-skeletal-muscle, antibody was diluted at 1:200)

Product Categories/Family for anti-TTN antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 378925625
• NCBI GeneID: 7273
• NCBI Accession #: NP_001243779.1
• NCBI GenBank Nucleotide #: NM_001256850.1
• UniProt Accession #: Q8WZ42; Q10465; Q10466; Q15598; Q2XUS3; Q32Q60; Q4U1Z6; Q4ZG20; A6NKB1; E7EQE6; E7ET18; K7ENY1
• Molecular Weight: 3,831,069 Da
• NCBI Official Full Name: titin isoform N2BA
• NCBI Official Synonym Full Names: titin
• NCBI Official Symbol: TTN
• NCBI Official Synonym Symbols: TMD; CMH9; CMD1G; CMPD4; EOMFC; HMERF; MYLK5; SALMY; LGMD2J
• NCBI Protein Information: titin
• UniProt Protein Name: Titin
• Protein Family: Titin
• UniProt Gene Name: TTN

NCBI Description

This gene encodes a large abundant protein of striated muscle. The product of this gene is divided into two regions, a N-terminal I-band and a C-terminal A-band. The I-band, which is the elastic part of the molecule, contains two regions of tandem immunoglobulin domains on either side of a PEVK region that is rich in proline, glutamate, valine and lysine. The A-band, which is thought to act as a protein-ruler, contains a mixture of immunoglobulin and fibronectin repeats, and possesses kinase activity. An N-terminal Z-disc region and a C-terminal M-line region bind to the Z-line and M-line of the sarcomere, respectively, so that a single titin molecule spans half the length of a sarcomere. Titin also contains binding sites for muscle associated proteins so it serves as an adhesion template for the assembly of contractile machinery in muscle cells. It has also been identified as a structural protein for chromosomes. Alternative splicing of this gene results in multiple transcript variants. Considerable variability exists in the I-band, the M-line and the Z-disc regions of titin. Variability in the I-band region contributes to the differences in elasticity of different titin isoforms and, therefore, to the differences in elasticity of different muscle types. Mutations in this gene are associated with familial hypertrophic cardiomyopathy 9, and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma. [provided by RefSeq, Feb 2012]

Uniprot Description

Titin: a protein kinase that is abundant in striated muscle. Is divided into two regions, a N-terminal I-band and a C-terminal A-band. The I-band, which is the elastic part of the molecule, contains two regions of tandem immunoglobulin domains on either side of a PEVK region that is rich in proline, glutamate, valine and lysine. The A-band, which is thought to act as a protein-ruler, contains a mixture of immunoglobulin and fibronectin repeats, and possesses kinase activity. A N-terminal Z-disc region and a C-terminal M-line region bind to the Z-line and M-line of the sarcomere respectively so that a single titin molecule spans half the length of a sarcomere. Titin also contains binding sites for muscle associated proteins so it serves as an adhesion template for the assembly of contractile machinery in muscle cells. It has also been identified as a structural protein for chromosomes. Mutations are associated with familial hypertrophic cardiomyopathy 9 and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma.

Protein type: CAMK group; EC 2.7.11.1; Kinase, protein; MLCK family; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 2q31.2

Cellular Component: condensed nuclear chromosome; cytosol; extracellular region; I band; M band; muscle myosin complex; Z disc

Molecular Function: actin filament binding; actinin binding; calcium ion binding; calmodulin binding; enzyme binding; identical protein binding; muscle alpha-actinin binding; protease binding; protein binding; protein kinase binding; protein self-association; protein serine/threonine kinase activity; protein-tyrosine kinase activity; structural constituent of muscle; telethonin binding

Biological Process: cardiac muscle contraction; cardiac muscle fiber development; cardiac muscle morphogensis; cardiac myofibril assembly; mitotic chromosome condensation; muscle contraction; muscle filament sliding; muscle thick filament assembly; muscle thin filament assembly; platelet degranulation; positive regulation of protein secretion; regulation of catalytic activity; regulation of protein kinase activity; response to calcium ion; sarcomere organization; sarcomerogenesis; striated muscle contraction

Disease: Cardiomyopathy, Dilated, 1g; Hereditary Myopathy With Early Respiratory Failure; Muscular Dystrophy, Limb-girdle, Type 2j; Myopathy, Early-onset, With Fatal Cardiomyopathy; Tibial Muscular Dystrophy, Tardive