Western Blot (WB)
(Western blot analysis of extracts of mouse testis tissue, using MTHFD2 antibody.)
Rabbit MTHFD2 Polyclonal Antibody | anti-MTHFD2 antibody
MTHFD2 Antibody
Gene Names
MTHFD2; NMDMC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MTHFD2; Polyclonal Antibody; MTHFD2 Antibody; Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase; mitochondrial; Descriptions; Methenyltetrahydrofolate cyclohydrolase; methylene tetrahydrofolate dehydrogenase (NAD+ dependent); methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2; methylenetetrahydrofolate dehydrogenase (NADP+ dependent); methylenetetrahydrofolate dehydrogenase 2; MGC82516; MTDC_HUMAN; mthfd2 methylene tetrahydrofolate dehydrogenase (NAD+ dependent); NAD-dependent methylene tetrahydrofolate dehydrogenase; NAD-dependent methylenetetrahydrofolate dehydrogenase; NMDMC; anti-MTHFD2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MTHFD2 antibody detects endogenous levels of MTHFD2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
350
Applicable Applications for anti-MTHFD2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human MTHFD2.
Subcellular Location
Mitochondrion.
Tissue Specificity
Expressed only in developing normal tissues.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (92%), Bovine (92%), Horse (92%), Sheep (92%), Rabbit (92%), Dog (92%), Chicken (83%), Xenopus (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of mouse testis tissue, using MTHFD2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of mouse testis tissue, using MTHFD2 antibody.)
Immunohistochemistry (IHC)
(MBS9603441 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603441 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-MTHFD2 antibody
Function: Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.
Subunit Structure: Homodimer.
Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
Subunit Structure: Homodimer.
Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 38 kDa
Predicted: 38 kDa
Predicted: 38 kDa
NCBI Official Full Name
bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
NCBI Official Synonym Full Names
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
NCBI Official Symbol
MTHFD2
NCBI Official Synonym Symbols
NMDMC
NCBI Protein Information
bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
UniProt Protein Name
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
UniProt Gene Name
MTHFD2
UniProt Synonym Gene Names
NMDMC
NCBI Description
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]
Uniprot Description
Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.
Research Articles on MTHFD2
Similar Products
Product Notes
The MTHFD2 mthfd2 (Catalog #AAA9603441) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MTHFD2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MTHFD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the MTHFD2 mthfd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTHFD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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