Western Blot (WB)
(Western Blot analysis of AKAP13 expression in transfected 293T cell line by AKAP13 polyclonal antibody. Lane 1: AKAP13 transfected lysate (120.2kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human AKAP13 Polyclonal Antibody | anti-AKAP13 antibody
AKAP13 (A-kinase Anchor Protein 13, AKAP-13, AKAP-Lbc, Breast Cancer Nuclear Receptor-binding Auxiliary Protein, Guanine Nucleotide Exchange Factor Lbc, Human Thyroid-anchoring Protein 31, Lymphoid Blast Crisis Oncogene, LBC Oncogene, Non-oncogenic Rho GT
Gene Names
AKAP13; BRX; LBC; HA-3; Ht31; c-lbc; AKAP-Lbc; ARHGEF13; FLJ11952; FLJ43341; PROTO-LB; PROTO-LBC; AKAP13
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
AKAP13; Polyclonal Antibody; AKAP13 (A-kinase Anchor Protein 13; AKAP-13; AKAP-Lbc; Breast Cancer Nuclear Receptor-binding Auxiliary Protein; Guanine Nucleotide Exchange Factor Lbc; Human Thyroid-anchoring Protein 31; Lymphoid Blast Crisis Oncogene; LBC Oncogene; Non-oncogenic Rho GT; anti-AKAP13 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human AKAP13.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-AKAP13 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human AKAP13, aa1-1058 (NP_658913.1).
Immunogen Sequence
MSSSKKSKEKEKEKDKIKEKEKDSKDKEKDKKTVNGHTFSSIPVVGPISCSQCMKPFTNKDAYTCANCSAFVHKGCRESLASCAKVKMKQPKGSLQAHDTSSLPTVIMRNKPSQPKERPRSAVLLVDETATTPIFANRRSQQSVSLSKSVSIQNITGVGNDENMSNTWKFLSHSTDSLNKISKVNESTESLTDEGVGTDMNEGQLLGDFEIESKQLEAESWSRIIDSKFLKQQKKDVVKRQEVIYELMQTEFHHVRTLKIMSGVYSQGMMADLLFEQQMVEKLFPCLDELISIHSQFFQRILERKKESLVDKSEKNFLIKRIGDVLVNQFSGENAERLKKTYGKFCGQHNQSVNYFKDLYAKDKRFQAFVKKKMSSSVVRRLGIPECILLVTQRITKYPVLFQRILQCTKDNEVEQEDLAQSLSLVKDVIGAVDSKVASYEKKVRLNEIYTKTDSKSIMRMKSGQMFAKEDLKRKKLVRDGSVFLKNAAGRLKEVQAVLLTDILVFLQEKDQKYIFASLDQKSTVISLKKLIVREVAHEEKGLFLISMGMTDPEMVEVHASSKEERNSWIQIIQDTINTLNRDEDEGIPSENEEEKKMLDTRARELKEQLHQKDQKILLLLEEKEMIFRDMAECSTPLPEDCSPTHSPRVLFRSNTEEALKGGPLMKSAINEVEILQGLVSGNLGGTLGPTVSSPIEQDVVGPVSLPRRAETFGGFDSHQMNASKGGEKEEGDDGQDLRRTESDSGLKKGGNANLVFMLKRNSEQVVQSVVHLYELLSALQGVVLQQDSYIEDQKLVLSERALTRSLSRPSSLIEQEKQRSLEKQRQDLANLQKQQAQYLEEKRRREREWEARERELREREALLAQREEEVQQGQQDLEKEREELQQKKGTYQYDLERLRAAQKQLEREQEQLRREAERLSQRQTERDLCQVSHPHTKLMRIPSFFPSPEEPPSPSAPSIAKSGSLDSELSVSPKRNSISRTHKDKGPFHILSSTSQTNKGPEGQSQAPASTSASTRLFGLTKPKEKKEKKKKNKTSRSQPGDGPASEVSAEGEEIFC
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of AKAP13 expression in transfected 293T cell line by AKAP13 polyclonal antibody. Lane 1: AKAP13 transfected lysate (120.2kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of AKAP13 expression in transfected 293T cell line by AKAP13 polyclonal antibody. Lane 1: AKAP13 transfected lysate (120.2kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-AKAP13 antibody
AKAP13 functions as a protein kinase A (PKA)-anchoring protein and acts as an adapter protein to selectively couple G alpha-13 and Rho. It contains a dbl oncogene homology (DH) domain and a pleckstrin homology (PH) domain. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins. AKAP13 augments gene activation by the estrogen receptor and activates estrogen receptor beta by a p38 MAPK-dependent pathway.
Product Categories/Family for anti-AKAP13 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
A-kinase anchor protein 13 isoform 3 [Homo sapiens]
NCBI Official Synonym Full Names
A kinase (PRKA) anchor protein 13
NCBI Official Symbol
AKAP13
NCBI Official Synonym Symbols
BRX; LBC; HA-3; Ht31; c-lbc; AKAP-Lbc; ARHGEF13; FLJ11952; FLJ43341; PROTO-LB; PROTO-LBC; AKAP13
NCBI Protein Information
A-kinase anchor protein 13; p47; AKAP-13; LBC oncogene; A-kinase anchoring protein; lymphoid blast crisis oncogene; human thyroid-anchoring protein 31; protein kinase A-anchoring protein 13; guanine nucleotide exchange factor Lbc; non-oncogenic Rho GTPase
UniProt Protein Name
A-kinase anchor protein 13
Protein Family
UniProt Gene Name
AKAP13
UniProt Synonym Gene Names
BRX; HT31; LBC; AKAP-13; LBC oncogene; PRKA13
UniProt Entry Name
AKP13_HUMAN
Uniprot Description
AKAP13: Anchors cAMP-dependent protein kinase (PKA) and acts as an adapter protein to selectively couple G alpha-13 and Rho. Augments gene activation by the estrogen receptor in an element- specific and ligand-dependent manner. Activates estrogen receptor beta by a p38 MAPK-dependent pathway. Stimulates exchange activity on Rho proteins in vitro, but not on CDC42, Ras or Rac and may bind calcium ions. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Nuclear receptor co-regulator; GEFs, Rac/Rho; Nuclear export; GEFs; Oncoprotein; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 15q24-q25
Cellular Component: membrane; perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol
Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity; signal transducer activity; Rho GTPase binding; cAMP-dependent protein kinase activity; metal ion binding; protein kinase A binding; protein complex scaffold
Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; nuclear export; regulation of protein kinase activity; protein amino acid phosphorylation
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Product Notes
The AKAP13 akap13 (Catalog #AAA6369246) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKAP13 (A-kinase Anchor Protein 13, AKAP-13, AKAP-Lbc, Breast Cancer Nuclear Receptor-binding Auxiliary Protein, Guanine Nucleotide Exchange Factor Lbc, Human Thyroid-anchoring Protein 31, Lymphoid Blast Crisis Oncogene, LBC Oncogene, Non-oncogenic Rho GT reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AKAP13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AKAP13 akap13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKAP13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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