Rabbit anti-Human TSG101 Polyclonal Antibody | anti-TSG101 antibody

Anti-TSG101 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101849

• Gene Names: TSG101; TSG10; VPS23
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: TSG101; Polyclonal Antibody; Anti-TSG101 Antibody; Rabbit Polyclonal; TSG101 Antibody; Rabbit PAb; Antigen Affinity Purified; tumor susceptibility 101; Anti-TSG10 Antibody; Anti-VPS23 Antibody; anti-TSG101 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human TSG101
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TSG101 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ICC/IF: 1:1500-1:50,000; IP: 0.2-1uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human TSG101
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human TSG101, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-TSG101 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: A431 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:43 kDaObserved band size:49 kDa(We are unsure as to the identity of these extra bands.))

Immunofluorescence (IF)

(Immunofluorescence staining of TSG101 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human TSG101 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.)

Immunoprecipitation (IP)

(TSG101 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate1 uL anti-TSG101 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TSG101 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size: 44 kDa)

Related Product Information for anti-TSG101 antibody

Tumor susceptibility gene 101 (TSG101) is a multi-functional gene involved in cell growth and proliferation in vertebrates. The tumor susceptibility gene 101 (TSG101) regulates AR expression level via the endosome/lysosome degradation pathway, that the interaction of the AR and TSG101 leads to androgen receptor (AR) recruitment to TSG101-containing cytoplasmic vesicles and induces AR-attenuated expression through the late endosome/lysosome degradation pathway. TSG101 elicits an array of cellular functions, including promoting cytokinesis, cell cycle progression and proliferation, as well as facilitating endosomal trafficking and viral budding.

NCBI and Uniprot Product Information

• NCBI GI #: 5454140
• NCBI GeneID: 7251
• NCBI Accession #: NP_006283.1
• NCBI GenBank Nucleotide #: NM_006292.3
• UniProt Accession #: Q99816; Q9BUM5
• Molecular Weight: 43,944 Da
• NCBI Official Full Name: tumor susceptibility gene 101 protein
• NCBI Official Synonym Full Names: tumor susceptibility gene 101
• NCBI Official Symbol: TSG101
• NCBI Official Synonym Symbols: TSG10; VPS23
• NCBI Protein Information: tumor susceptibility gene 101 protein; tumor susceptibility protein; ESCRT-I complex subunit TSG101
• UniProt Protein Name: Tumor susceptibility gene 101 protein
• Protein Family: Tumor susceptibility gene 101 protein
• UniProt Gene Name: TSG101
• UniProt Entry Name: TS101_HUMAN

NCBI Description

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]

Uniprot Description

TSG101: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: multivesicular body; late endosome membrane; early endosome; cytoplasm; late endosome; plasma membrane; nucleolus; endosome membrane

Molecular Function: ubiquitin binding; protein binding; protein homodimerization activity; DNA binding; ubiquitin protein ligase binding; transcription corepressor activity; calcium-dependent protein binding

Biological Process: viral reproduction; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; non-lytic virus budding; protein modification process; viral infectious cycle; virus assembly; endosome transport; keratinocyte differentiation; negative regulation of cell proliferation; protein transport; cell division; viral protein processing; regulation of cell growth; cell cycle arrest; negative regulation of transcription, DNA-dependent

Disease: Breast Cancer