Rabbit anti-Human, Mouse TSG101 Polyclonal Antibody | anti-TSG101 antibody

TSG101, NT (Tumor Susceptibility Gene 101 Protein, ESCRT-I Complex Subunit TSG101) (AP)

Cat. Num. AAA6503995

• Gene Names: TSG101; TSG10; VPS23
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: TSG101; Polyclonal Antibody; NT (Tumor Susceptibility Gene 101 Protein; ESCRT-I Complex Subunit TSG101) (AP); anti-TSG101 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TSG101. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TSG101 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human TSG101, UniProt Accession #Q99816.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot to detect TSG101 in mouse kidney tissue lysate using T9109-75A.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue using T9109-75A, followed by peroxidase-conjugated to the secondary antibody, followed by AEC staining.)

Related Product Information for anti-TSG101 antibody

TSG101 belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.

Product Categories/Family for anti-TSG101 antibody

Antibodies; Disease Markers

References

1. Favre, M., et al., J. Acquir. Immune Defic. Syndr. 34(2):127-133 (2003). 2. Lu, Q., et al., Proc. Natl. Acad. Sci. USA 100(13):7626-7631 (2003). 3. Goila-Gaur, R., et al., J. Virol. 77(11):6507-6519 (2003). 4. Blanco, S., et al., FEMS Microbiol. Lett. 221(2):151-154 (2003). 5. Martin-Serrano, J., et al., J. Virol. 77(8):4794-4804 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 5454140
• NCBI GeneID: 7251
• NCBI Accession #: NP_006283.1
• NCBI GenBank Nucleotide #: NM_006292.3
• UniProt Accession #: Q99816; Q9BUM5
• Molecular Weight: 43,944 Da
• NCBI Official Full Name: tumor susceptibility gene 101 protein
• NCBI Official Synonym Full Names: tumor susceptibility gene 101
• NCBI Official Symbol: TSG101
• NCBI Official Synonym Symbols: TSG10; VPS23
• NCBI Protein Information: tumor susceptibility gene 101 protein; tumor susceptibility protein; ESCRT-I complex subunit TSG101
• UniProt Protein Name: Tumor susceptibility gene 101 protein
• Protein Family: Tumor susceptibility gene 101 protein
• UniProt Gene Name: TSG101
• UniProt Entry Name: TS101_HUMAN

NCBI Description

The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]

Uniprot Description

TSG101: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Cell cycle regulation

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: multivesicular body; late endosome membrane; early endosome; cytoplasm; late endosome; plasma membrane; nucleolus; endosome membrane

Molecular Function: ubiquitin binding; protein binding; protein homodimerization activity; DNA binding; ubiquitin protein ligase binding; transcription corepressor activity; calcium-dependent protein binding

Biological Process: viral reproduction; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; non-lytic virus budding; protein modification process; viral infectious cycle; virus assembly; endosome transport; keratinocyte differentiation; negative regulation of cell proliferation; protein transport; cell division; viral protein processing; regulation of cell growth; cell cycle arrest; negative regulation of transcription, DNA-dependent

Disease: Breast Cancer