Rabbit anti-Human TSC2 Polyclonal Antibody | anti-TSC2 antibody

TSC2, Phosphorylated (Ser1798) (Tuberin, Tuberous Sclerosis 2 Protein, TSC4) (FITC)

Cat. Num. AAA6503943

• Gene Names: TSC2; LAM; TSC4; PPP1R160
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: TSC2; Polyclonal Antibody; Phosphorylated (Ser1798) (Tuberin; Tuberous Sclerosis 2 Protein; TSC4) (FITC); anti-TSC2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TSC2 phosphorylated at Ser1798.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TSC2 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser1798 of human TSC2 (KLH).
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of Phospho-TSC2-S1798 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Dot Blot (DB)

(Dot blot analysis of anti-TSC2-pS1798 Phospho-specific Pab (RB13393) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-TSC2 antibody

Antibodies; Tumor Suppressors

References

1. Li, Y., et al., Mol. Cell. Biol. 24(18):7965-7975 (2004). 2. Karbowniczek, M., et al., J. Biol. Chem. 279(29):29930-29937 (2004). 3. Corradetti, M.N., et al., Genes Dev. 18(13):1533-1538 (2004). 4. Birchenall-Roberts, M.C., et al., J. Biol. Chem. 279(24):25605-25613 (2004). 5. Lewis, J.C., et al., J. Med. Genet. 41(3):203-207 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 116256352
• NCBI GeneID: 7249
• NCBI Accession #: NP_000539
• NCBI GenBank Nucleotide #: NM_000548.3
• UniProt Accession #: P49815; O75275; Q4LE71; Q8TAZ1; A7E2E2; B4DIL8; B4DIQ7; B4DRN2; B7Z2B8; C9J378
• Molecular Weight: 25,773 Da
• NCBI Official Full Name: tuberin isoform 1
• NCBI Official Synonym Full Names: tuberous sclerosis 2
• NCBI Official Symbol: TSC2
• NCBI Official Synonym Symbols: LAM; TSC4; PPP1R160
• NCBI Protein Information: tuberin; tuberous sclerosis 2 protein; protein phosphatase 1, regulatory subunit 160
• UniProt Protein Name: Tuberin
• Protein Family: Tuberin
• UniProt Gene Name: TSC2
• UniProt Synonym Gene Names: TSC4
• UniProt Entry Name: TSC2_HUMAN

NCBI Description

Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TSC2: a product of the tumor suppressor gene TSC2. Tuberin and Hamartin (TSC1) form a tumor suppressor heterodimer that inhibits the mTOR nutrient signaling input. TSC1/TSC2 targets the small G protein Rheb, a novel mediator of the nutrient signaling input to mTOR. Functions as a Rheb GTPase activating protein (GAP). Four alternatively spliced isoforms have been described.

Protein type: Tumor suppressor; GAPs, misc.; Cell cycle regulation; Nuclear receptor co-regulator; GAPs

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: TSC1-TSC2 complex; Golgi apparatus; cytoskeleton; growth cone; membrane; cell soma; perinuclear region of cytoplasm; cytoplasm; dendrite; caveola; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; GTPase activator activity; phosphatase binding

Biological Process: negative regulation of MAP kinase activity; phosphoinositide 3-kinase cascade; regulation of insulin receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; nerve growth factor receptor signaling pathway; protein heterooligomerization; regulation of cell cycle; heart development; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; negative regulation of cell proliferation; protein localization; positive chemotaxis; cell projection organization and biogenesis; protein import into nucleus; regulation of endocytosis; acute-phase response; protein kinase B signaling cascade; cell cycle arrest; protein homooligomerization; negative regulation of epithelial cell proliferation; epidermal growth factor receptor signaling pathway; negative regulation of TOR signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of protein kinase B signaling cascade; endocytosis; vesicle-mediated transport; insulin-like growth factor receptor signaling pathway; glucose import; neural tube closure; insulin receptor signaling pathway; response to hypoxia; innate immune response; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of phosphoinositide 3-kinase cascade

Disease: Lymphangioleiomyomatosis; Tuberous Sclerosis 2