Rabbit anti-Human TRPM8 Polyclonal Antibody | anti-TRPM8 antibody

TRPM8, CT (C940) (Transient Receptor Potential Cation Channel Subfamily M Member 8, Long Transient Receptor Potential Channel 6, LTrpC-6, LTrpC6, Transient Receptor Potential-p8, Trp-p8, LTRPC6, TRPP8) (APC)

Cat. Num. AAA6503849

• Gene Names: TRPM8; TRPP8; LTRPC6
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: TRPM8; Polyclonal Antibody; CT (C940) (Transient Receptor Potential Cation Channel Subfamily M Member 8; Long Transient Receptor Potential Channel 6; LTrpC-6; LTrpC6; Transient Receptor Potential-p8; Trp-p8; LTRPC6; TRPP8) (APC); anti-TRPM8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRPM8.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TRPM8 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide at the C-term(C940) of human TRPM8 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TRPM8 (arrow) using rabbit polyclonal TRPM8 Antibody (C-term C940).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRPM8 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TRPM8 antibody (C-term C940), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-TRPM8 antibody

Antibodies; Ion Channel

References

1. Tsavaler L., Cancer Res. 61:3760-3769(2001). 2. Kiessling A., Prostate 56:270-279(2003). 3. Bodding, M., Cell Calcium 42(6), 618-628 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 109689695
• NCBI GeneID: 79054
• NCBI Accession #: NP_076985
• NCBI GenBank Nucleotide #: NM_024080
• UniProt Accession #: Q7Z2W7; Q3YFM7; Q6QNH9; Q8TAC3; Q8TDX8; Q9BVK1; A0AVG2
• Molecular Weight: 79,096 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 8
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily M, member 8
• NCBI Official Symbol: TRPM8
• NCBI Official Synonym Symbols: TRPP8; LTRPC6
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 8; LTrpC-6; long transient receptor potential channel 6; short form of the TRPM8 cationic channel; transient receptor melastatin 8 variant 1; transient receptor melastatin 8 variant 2; transie
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 8
• Protein Family: TRPM8 channel-associated factor
• UniProt Gene Name: TRPM8
• UniProt Synonym Gene Names: LTRPC6; TRPP8; LTrpC-6; LTrpC6; Trp-p8
• UniProt Entry Name: TRPM8_HUMAN

Uniprot Description

TRPM8: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Channel, cation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: endoplasmic reticulum membrane; external side of plasma membrane; integral to membrane; lipid raft; plasma membrane

Molecular Function: calcium channel activity; protein binding; protein homodimerization activity

Biological Process: cellular calcium ion homeostasis; detection of temperature stimulus; protein homotetramerization; response to cold; thermoception