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SDS-Page

Plexin B1 Recombinant Protein | PLXNB1 recombinant protein

Recombinant Plexin B1 (PLXNB1)

Gene Names
PLXNB1; SEP; PLXN5; PLEXIN-B1
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>95%
Synonyms
Plexin B1; Recombinant Plexin B1 (PLXNB1); PLXNB1 recombinant protein
Ordering
For Research Use Only!
Host
Host: E Coli
Source: Prokaryotic expression
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
LFL TKFIHTLESQ RTFSARDRAY VASLLTVALH GKLEYFTDIL RTLLSDLVAQ YVAKNPKLML RRTETVVEKL LTNWMSICLY TFVRDSVGEP LYMLFRGIKH QVDKGPVDSV TGKAKYTLND NRLLREDVEY RPLTLNALLA VGPGAGEAQC VPVKVLDCDT ISQAKEKMLD QLYKGVPLAQ RPDSCTLDVE WRSGVAGHLI LSDEDVTSEL QGLWRRLNTL QHYKVPDGAT VALVPCLTKHILRENQDYV
Sequence Length
2135
Applicable Applications for PLXNB1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
32.1kDa
Accurate Molecular Mass (KD)
30kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
N-terminal His-Tag, T7 tag
Organism Species
Mus musculus (Mouse)
Fragment
Leu1598~Val1849
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
78,395 Da
NCBI Official Full Name
plexin-B1
NCBI Official Synonym Full Names
plexin B1
NCBI Official Symbol
PLXNB1
NCBI Official Synonym Symbols
SEP; PLXN5; PLEXIN-B1
NCBI Protein Information
plexin-B1
UniProt Protein Name
Plexin-B1
Protein Family
UniProt Gene Name
PLXNB1
UniProt Synonym Gene Names
KIAA0407; PLXN5; SEP
UniProt Entry Name
PLXB1_HUMAN

Uniprot Description

PLXNB1: Receptor for SEMA4D. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Belongs to the plexin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: integral to plasma membrane; plasma membrane; extracellular region; intracellular

Molecular Function: protein binding; transmembrane receptor activity; semaphorin receptor activity; GTPase activating protein binding; receptor activity; semaphorin receptor binding

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; axon guidance; regulation of cell shape; cell migration; positive regulation of axonogenesis; negative regulation of osteoblast proliferation; negative regulation of cell adhesion; signal transduction; neurite morphogenesis; regulation of cytoskeleton organization and biogenesis

Research Articles on PLXNB1

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Product Notes

The PLXNB1 plxnb1 (Catalog #AAA2029252) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Plexin B1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the PLXNB1 plxnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
LFL TKFIHTLESQ RTFSARDRAY VASLLTVALH GKLEYFTDIL RTLLSDLVAQ YVAKNPKLML RRTETVVEKL LTNWMSICLY TFVRDSVGEP LYMLFRGIKH QVDKGPVDSV TGKAKYTLND NRLLREDVEY RPLTLNALLA VGPGAGEAQC VPVKVLDCDT ISQAKEKMLD QLYKGVPLAQ RPDSCTLDVE WRSGVAGHLI LSDEDVTSEL QGLWRRLNTL QHYKVPDGAT VALVPCLTKH ILRENQDYV. It is sometimes possible for the material contained within the vial of "Plexin B1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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