Rabbit anti-Human TRPM4 Polyclonal Antibody | anti-TRPM4 antibody

TRPM4, CT (TRPM4, LTRPC4, Transient receptor potential cation channel subfamily M member 4, Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4, Melastatin-4) (FITC)

Cat. Num. AAA6358861

• Gene Names: TRPM4; LTrpC4; PFHB1B; TRPM4B; hTRPM4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRPM4; Polyclonal Antibody; CT (TRPM4; LTRPC4; Transient receptor potential cation channel subfamily M member 4; Calcium-activated non-selective cation channel 1; Long transient receptor potential channel 4; Melastatin-4) (FITC); Melastatin-4; anti-TRPM4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRPM4.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TRPM4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1121-1149 in the C-terminal region of human TRPMR, UniProt Accession #Q8TD43.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS641161 in 293 cell line lysates (35ug/lane). This demonstrates the TRPM4 antibody detected the TRPM4 protein (arrow).)

Related Product Information for anti-TRPM4 antibody

The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-TRPM4 antibody

Antibodies; Receptors

References

1. Liu, H., et al. Circ Cardiovasc Genet 3(4):374-385 (2010). 2. Yoo, J.C., et al. Biochem. Biophys. Res. Commun. 391(1):806-811 (2010). 3. Kruse, M., et al. J. Clin. Invest. 119(9):2737-2744 (2009). 4. Marigo, V., et al. Mol. Cell. Endocrinol. 299(2):194-203 (2009). 5. Park, J.Y., et al. Biochem. Biophys. Res. Commun. 368(3):677-683 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 304766677
• NCBI GeneID: 54795
• NCBI Accession #: NP_001182156.1; NP_060106.2
• NCBI GenBank Nucleotide #: NM_001195227.1
• UniProt Accession #: Q8TD43; Q7Z5D9; Q96L84; Q9NXV1; A2RU25
• Molecular Weight: 118,630 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 4 isoform 2
• NCBI Official Synonym Full Names: transient receptor potential cation channel subfamily M member 4
• NCBI Official Symbol: TRPM4
• NCBI Official Synonym Symbols: LTrpC4; PFHB1B; TRPM4B; hTRPM4
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 4
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 4
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: TRPM4
• UniProt Synonym Gene Names: LTRPC4; hTRPM4; LTrpC-4; LTrpC4
• UniProt Entry Name: TRPM4_HUMAN

NCBI Description

The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium. [provided by RefSeq, Mar 2016]

Uniprot Description

TRPM4: Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway. Defects in TRPM4 are the cause of progressive familial heart block type 1B (PFHB1B). It is a cardiac bundle branch disorder characterized by progressive alteration of cardiac conduction through the His-Purkinje system, with a pattern of a right bundle-branch block and/or left anterior hemiblock occurring individually or together. It leads to complete atrioventricular block causing syncope and sudden death. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM4 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: nucleoplasm; Golgi apparatus; endoplasmic reticulum; plasma membrane; integral to membrane

Molecular Function: calmodulin binding; calcium channel activity; calcium activated cation channel activity; ATP binding

Biological Process: vasoconstriction; regulation of membrane potential; protein sumoylation; regulation of T cell cytokine production; positive regulation of cell proliferation; dendritic cell chemotaxis; transmembrane transport

Disease: Progressive Familial Heart Block, Type Ib