Western Blot (WB)
(Western blot analysis of TRPC3 in human cerebellum tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 μg/ml.)
Rabbit TRPC3 Polyclonal Antibody | anti-TRPC3 antibody
TRPC3 Antibody
Gene Names
TRPC3; TRP3; SCA41
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity chromatography purified via peptide column.
Synonyms
TRPC3; Polyclonal Antibody; TRPC3 Antibody; Transient receptor potential cation channel; subfamily C; member 3; TRP3; Short transient receptor potential channel 3; Transient receptor protein 3; TrpC3; anti-TRPC3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TRPC3 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect the three largest isoforms. TRPC3 antibody is predicted to not cross-react with TRPC6.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
921
Applicable Applications for anti-TRPC3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 mug/mL.Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL.
Immunogen
TRPC3 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human TRPC3.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
Human Cerebellum Tissue Lysate
Conjugate
Unconjugated
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of TRPC3 in human cerebellum tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 μg/ml.)
Western Blot (WB)
(Western blot analysis of TRPC3 in human cerebellum tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of TRPC3 in human brain tissue with TRPC3 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of TRPC3 in human brain tissue with TRPC3 antibody at 2.5 μg/mL.)
Related Product Information for anti-TRPC3 antibody
The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC3/6/7 subfamily can be activated by diacylglycerol analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. TRPC3 encodes a Ca2+-permeant channel that is agonist-activated but not store-operated or directly receptor-activated. TRPC3 physically interacts with TRPC6 and TRPC7 and forms functional tetrameric channels.
Product Categories/Family for anti-TRPC3 antibody
References
Contell C, Birnbaumer V, Flockerzi V, et al. A unified nomenclature for the superfamily of TRP cation channels. Mol. Cell 2002; 9:229-31. Trebak M, Vazquez G, Bird GSJ, et al. The TRPC3/6/7 subfamily of cation channels. Cell Calcium 2003; 33:451-61. Zitt C, Obukhov AG, Strubing C, et al. Expression of TRPC3 in Chinese hamster ovary cells results in calcium-activated cation currents not related to store depletion. J. Cell. Biol. 1997; 1333-41. Dietrich A, Kalwa H, Rost BR, et al. The diacylglycerol-sensitive TRPC3/6/7 subfamily of cation channels: functional characterization and physiological relevance. Pflugers Arch. 2005; 451:72-80.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 90, 93, 101 kDa
Observed: 93 kDa
Observed: 93 kDa
NCBI Official Full Name
short transient receptor potential channel 3 isoform a
NCBI Official Synonym Full Names
transient receptor potential cation channel subfamily C member 3
NCBI Official Symbol
TRPC3
NCBI Official Synonym Symbols
TRP3; SCA41
NCBI Protein Information
short transient receptor potential channel 3
UniProt Protein Name
Short transient receptor potential channel 3
Protein Family
UniProt Gene Name
TRPC3
UniProt Synonym Gene Names
TRP3; TrpC3; TRP-3; hTrp-3; hTrp3
UniProt Entry Name
TRPC3_HUMAN
NCBI Description
The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol 1,4,5-triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion.
Research Articles on TRPC3
Similar Products
Product Notes
The TRPC3 trpc3 (Catalog #AAA154368) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRPC3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRPC3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 mug/mL.Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. Researchers should empirically determine the suitability of the TRPC3 trpc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRPC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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