Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat TNNI3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Rat Troponin I Type 3, Cardiac (TNNI3) Polyclonal Antibody | anti-TNNI3 antibody
Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)
MGHHHHHHSGS-MADESSDAAG EPQPAPAPVR RRSSANYRAY ATEPHAKKKS KISASRKLQL KTLMLQIAKQ EMEREAEERR GEKGRVLSTR CQPLVLDGLG FEELQDLCRQ LHARVDKVDE ERYDVEAKVT KNITEIADLT QKIYDLRGKF KRPTLRRVRI SADAMMQALL GTRAKESLDL RAHLKQVKKE DIEKENREVG DWRKNIDALS GMEGRKKKFE G
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat TNNI3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat TNNI3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Intestine Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Intestine Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Heart Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Heart Tissue))
NCBI and Uniprot Product Information
NCBI Description
cardiac isoform of troponin I, which is a subunit of the troponin complex; inhibits actomyosin ATPase activity in muscle conraction [RGD, Feb 2006]
Uniprot Description
TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.
Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor
Chromosomal Location of Human Ortholog: 1q12
Cellular Component: cardiac Troponin complex; contractile fiber; cytoplasm; myofibril; sarcomere; troponin complex
Molecular Function: actin binding; actin filament binding; calcium channel inhibitor activity; calcium-dependent protein binding; metal ion binding; protein binding; protein domain specific binding; protein kinase binding; troponin C binding; troponin T binding
Biological Process: cardiac muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; negative regulation of ATPase activity; regulation of cardiac muscle contraction by calcium ion signaling; regulation of muscle contraction; regulation of smooth muscle contraction; regulation of systemic arterial blood pressure by ischemic conditions; skeletal muscle contraction; striated muscle contraction; vasculogenesis; ventricular cardiac muscle tissue morphogenesis
Research Articles on TNNI3
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Product Notes
The TNNI3 tnni3 (Catalog #AAA2015291) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Troponin I Type 3, Cardiac (TNNI3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TNNI3 tnni3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG S-MADESSDA AG EPQPAPAPVR RRSSANYRAY ATEPHAKKKS KISASRKLQL KTLMLQIAKQ EMEREAEERR GEKGRVLSTR CQPLVLDGLG FEELQDLCRQ LHARVDKVDE ERYDVEAKVT KNITEIADLT QKIYDLRGKF KRPTLRRVRI SADAMMQALL GTRAKESLDL RAHLKQVKKE DIEKENREVG DWRKNIDALS GMEGRKKKFE G. It is sometimes possible for the material contained within the vial of "Troponin I Type 3, Cardiac (TNNI3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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