Rat Troponin I ELISA Kit | Tnni3 elisa kit

Rat Troponin I, cardiac muscle ELISA Kit

Cat. Num. AAA9425677

• Gene Names: Tnni3; TnI; cTNI
• Reactivity: Rat
• Synonyms: Troponin I; Rat Troponin I; cardiac muscle ELISA Kit; cardiac muscle; Cardiac troponin I; Tnni3; Ctni; Tni; Tnni3 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat Troponin I, cardiac muscle. No significant cross-reactivity or interference was observed. Note?Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between rat Troponin I, cardiac muscle and all the analogues, therefore, cross reaction may still exist.

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
• Detection Range: 1.25 ng/mL -80.0 ng/mL.
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Tnni3 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of rat Troponin I, cardiac muscle concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin I, cardiac muscle. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Troponin I, cardiac muscle and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Troponin I, cardiac muscle, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Troponin I, cardiac muscle in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8394469
• NCBI GeneID: 29248
• NCBI Accession #: NP_058840.1
• NCBI GenBank Nucleotide #: NM_017144.2
• UniProt Accession #: P23693; Q4PP23
• Molecular Weight: 24,160 Da
• NCBI Official Full Name: troponin I, cardiac muscle
• NCBI Official Synonym Full Names: troponin I3, cardiac type
• NCBI Official Symbol: Tnni3
• NCBI Official Synonym Symbols: TnI; cTNI
• NCBI Protein Information: troponin I, cardiac muscle
• UniProt Protein Name: Troponin I, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: Tnni3
• UniProt Synonym Gene Names: Ctni; Tni

NCBI Description

cardiac isoform of troponin I, which is a subunit of the troponin complex; inhibits actomyosin ATPase activity in muscle conraction [RGD, Feb 2006]

Uniprot Description

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 1q12

Cellular Component: contractile fiber; cytoplasm; myofibril; sarcomere; troponin complex

Molecular Function: actin binding; actin filament binding; calcium channel inhibitor activity; calcium-dependent protein binding; protein binding; protein domain specific binding; protein kinase binding; troponin C binding; troponin T binding

Biological Process: cardiac muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; negative regulation of ATPase activity; regulation of muscle contraction; regulation of smooth muscle contraction; regulation of systemic arterial blood pressure by ischemic conditions; skeletal muscle contraction; striated muscle contraction; vasculogenesis; ventricular cardiac muscle morphogenesis